中国中药杂志

2004, (11)

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高效液相色谱法测定香薷药材中香芹酚和百里香酚的含量
Determination of carvacrol and thymol in Mosla chinensis by HPLC

吉力,汪芳,刘元艳,仝燕,李先端,冯学峰,黄璐琦,周国平
JI Li1, WANG Fang1, LIU Yuan-yan1, TONG Yan1, LI Xian- duan1, FENG Xue-feng1, HUANG Lu-qi1, ZHOU Guo-ping2 (1. Institute of Chinese Materia Medica, China Academy of Tradition al Chinese Medicine, Beijing 100700, China; 2. Jiangxi provincial Institute for Drug Control, Nanchang 330000, China) [

摘要(Abstract):

目的 :建立香薷药材中香芹酚和百里香酚的含量测定方法。方法 :用高效液相色谱法 ,95 %乙醇超声提取 ,色谱柱InertsilODS 3,流动相甲醇 水 冰醋酸 (6 0∶4 0∶2 ) ,检测波长 2 74nm。结果 :香芹酚在 0 2 3~ 2 15 μg (r=0 9999) ,百里香酚在 0 39~ 2 36 μg (r =0 9999)有良好的线性关系 ;平均回收率分别为香芹酚 99 9% (RSD1 4 % ) ,百里香酚 98 6 % (RSD 1 3% ) ,重复性的RSD分别为香芹酚 1 1% ,百里香酚 1 6 %。结论 :该方法可靠、便捷 ,可以作为控制香薷药材质量的方法。
Objective: To establish a quantitative method o f determination of carvacrol and thymol in Mosla chinensis. Method: The sample was extracted with 95% ethanol, ODS column was used with met hanol-water-acetic acid (60∶40∶2) as mibile phase. The detection wavelength was set at 274 nm. Result: The linearities of carvacrol and thymol were respectively in the range of 0.23~2.15 μg (r=0.999 9) and 0.39~2.36 μg (r=0.999 9); the average recoveries were 99.9% (RSD 1.4%) and 98.6% (RSD 1.3%); the RSD of repeatability were 1.1% and 1.6% . Conclusion: The mothod is reliable, and can be used for qu ality control of M. chinensis. [

关键词(KeyWords): 石香薷;香芹酚;百里香酚;高效液相色谱法
Mosla chinensis; carvacrol; thymol; HPLC

Abstract:

Keywords:

基金项目(Foundation): 中央级科研院所科技基础性工作专项资金

作者(Author): 吉力,汪芳,刘元艳,仝燕,李先端,冯学峰,黄璐琦,周国平
JI Li1, WANG Fang1, LIU Yuan-yan1, TONG Yan1, LI Xian- duan1, FENG Xue-feng1, HUANG Lu-qi1, ZHOU Guo-ping2 (1. Institute of Chinese Materia Medica, China Academy of Tradition al Chinese Medicine, Beijing 100700, China; 2. Jiangxi provincial Institute for Drug Control, Nanchang 330000, China) [

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