中国中药杂志

2018, v.43(07) 1441-1445

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开发indel分子标记对人参与西洋参的鉴别研究
Development of indel markers for molecular authentication of Panax ginseng and P. quinquefolius

王戎博;田惠丽;王洪涛;李桂生;
WANG Rong-bo;TIAN Hui-li;WANG Hong-tao;LI Gui-sheng;School of Pharmacy,Yantai University;College of Life Science,Yantai University;

摘要(Abstract):

人参和西洋参是2种重要的中药材。二者虽然形态相似但药理作用不同。因此,准确地鉴别2种药材对于确保用药及食品安全具有非常重要的意义。该研究根据垂直同源基因中内含子位置相对保守的特点,利用人参的unigene开发了人参和西洋参之间的内含子长度多态性标记。基于人参及西洋参在细胞色素P450和甘油醛-3-磷酸脱氢酶基因中的插入/缺失序列,分别设计了人参和西洋参的特异性引物,并以此建立了用于人参及西洋参鉴别的多重PCR体系。结果表明,该研究建立的多重PCR体系既不需要严格的PCR条件,也无需对反应体系进行优化,可以对人参及西洋参进行有效地区分和鉴定。因此,该研究为人参及西洋参的植物来源鉴定提供了的理想的分子标记系统,也为其他中药材或功能食品的原料来源鉴定提供了方法借鉴。
Panax ginseng and P. quinquefolius are two kinds of important medicinal herbs. They are morphologically similar but have different pharmacological effects. Therefore,botanical origin authentication of these two ginsengs is of great importance for ensuring pharmaceutical efficacy and food safety. Based on the fact that intron position in orthologous genes is highly conserved across plant species,intron length polymorphisms were exploited from unigenes of ginseng. Specific primers were respectively designed for these two species based on their insertion/deletion sequences of cytochrome P450 and glyceraldehyde 3-phosphate dehydrogenase,and multiplex PCR was conducted for molecular authentication of P. ginseng and P. quinquefolius. The results showed that the developed multiplex PCR assay was effective for molecular authentication of P. ginseng and P. quinquefolius without strict PCR condition and the optimization of reaction system. This study provides a preferred ideal marker system for molecular authentication of ginseng,and the presented method can be employed in origin authentication of other herbal preparations.

关键词(KeyWords): 人参;西洋参;插入/缺失标记;内含子;多重PCR
Panax ginseng;Panax quinquefolius;indel marker;intron;multiplex PCR

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(81503178)

作者(Author): 王戎博;田惠丽;王洪涛;李桂生;
WANG Rong-bo;TIAN Hui-li;WANG Hong-tao;LI Gui-sheng;School of Pharmacy,Yantai University;College of Life Science,Yantai University;

Email:

DOI: 10.19540/j.cnki.cjcmm.20180201.004

参考文献(References):

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