中国中药杂志

2013, v.38(21) 3713-3719

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黄连HPLC数字化指纹图谱研究及7种生物碱含量测定
Study on HPLC digital fingerprint of Coptidis Rhizoma and content determination of seven alkaloids

刘芳;张浩;青琳森;
LIU Fang;ZHANG Hao;QING Lin-sen;Changzhi Medical College;West China School of Pharmacy,Sichuan University;Chengdu Institute of Biology,Chinese Academy of Sciences;

摘要(Abstract):

该文建立了峨眉地区道地药材黄连的HPLC数字化指纹图谱,并将其在全国不同主产区的黄连测定中进行了应用,同时测定黄连中7种季胺型生物碱的含量,为科学评价与有效控制黄连质量提供依据。采用Diamonsil C18色谱柱(4.6mm×250 mm,5μm),流动相乙腈-0.025mol·L-1KH2PO4溶液(磷酸调pH 3.0)(40∶60),内含SDS 1.7 g·L-1,流速1.2 mL·min-1,检测波长345 nm,柱温40℃。运用中药色谱指纹图谱相似度评价系统(国家药典委员会)进行分析,10批次峨眉产黄连样品得到的指纹图谱相似度均大于0.99,标定共有峰10个,并对其中8个色谱峰进行了归属。全国5个黄连主产区样品与峨眉产黄连进行相似度分析比较,整体相似度高。同时对黄连药材中groenlandicine,非洲防己碱,药根碱,表小檗碱,黄连碱,巴马汀,小檗碱7种主要生物碱进行了含量测定。该方法灵敏度高、专属性强,HPLC指纹图谱结合生物碱含量测定能全面反应其内在质量,为黄连的质量控制提供科学依据。
To establish an HPLC digital fingerprint for Rhizoma Coptidis produced in E'mei,and apply it in the determination of Rhizoma Coptidis herbs produced in main producing areas nationwide,while determining the content of seven quaternary ammonium alkaloids contained in Coptidis Rhizoma,in order to provide basis for scientific evaluation and effective control over quality of Rhizoma Coptidis. Diamonsil C 18 column(4. 6 mm × 250 mm,5μm) was adopted and eluted with acetonitrile 0. 025 mol·L- 1KH 2 PO 4 solution(adjust pH to 3. 0 with H 3 PO 4)(40∶ 60) containing SDS 1. 7 g·L- 1. The flow rate was 1. 2 mL·min- 1,the detective wavelength was 345 nm,and the column temperature was 40 ℃. The chromatographic fingerprint similarity evaluation system for traditional Chinese medicines(state pharmacopeia committee of China) was used for analysis. The chromatographic fingerprint similarity of ten batches of Coptidis Rhizoma samples produced in Emei was more than 0. 99. There were altogether 10 common peaks,and eight of them were identified. A comparative analysis showed a high similarity between samples from five main producing areas nationwide and Coptidis Rhizoma herbs in E'mei. Meanwhile,the content of seven major alkaloids contained in Coptidis Rhizoma herbs,namely groenlandicine,columbamine,jatrorrhizine,epiberberine,coptisine,palmatine and berberine were determined. As the method was highly sensitive and specific,the combination of HPLC digital fingerprint and alkaloid content determination could reflect its inherent quality in an all-round way,which provided scientific basis for the quality control of Coptidis Rhizoma.

关键词(KeyWords): 黄连;HPLC;指纹图谱;含量测定
Coptidis Rhizoma;high performance liquid chromatography;fingerprint;content determination

Abstract:

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基金项目(Foundation): 科技部国家科技支撑计划重点项目(2007BA140B05);; 中国博士后科学基金第6批特别资助项目(2013T60863)

作者(Author): 刘芳;张浩;青琳森;
LIU Fang;ZHANG Hao;QING Lin-sen;Changzhi Medical College;West China School of Pharmacy,Sichuan University;Chengdu Institute of Biology,Chinese Academy of Sciences;

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