中国中药杂志

2018, v.43(01) 92-99

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瓜蒌与瓜蒌条抗大鼠心肌缺血-再灌注损伤的谱效关系研究
Spectrum-activity relationship of trichosanthis fructus and trichosanthis fructus strip pieces for rat myocardial ischemia-reperfusion injury

邹纯才;宗倩妮;鄢海燕;
ZOU Chun-cai;ZONG Qian-ni;YAN Hai-yan;Pharmacy School of Wannan Medical College;

摘要(Abstract):

研究瓜蒌与瓜蒌条抗大鼠心肌缺血-再灌注损伤的谱效关系。建立瓜蒌与瓜蒌条的HPLC指纹图谱,测定瓜蒌与瓜蒌条3个剂量组(2.25,13.5,27.0 g·kg-1,相当于生药g·kg-1)抗大鼠心肌缺血-再灌注损伤的肌酸激酶同工酶(creatinekinaseMB,CK-MB)、心肌肌红蛋白(myoglobin,MYO)、心肌肌钙蛋白-T(cardiac troponin-T,c TNT)值,采用灰关联度法研究瓜蒌与瓜蒌条抗大鼠心肌缺血-再灌注损伤的谱效关系。给药剂量由2.25 g·kg-1增加到27.0 g·kg-1,瓜蒌与瓜蒌条抗大鼠心肌缺血-再灌注损伤的谱效关联的关联度也增强,但瓜蒌与瓜蒌条的变化趋势不同。按关联度前10出现频率排序,17,14,16,19,32,12,26,30,4,6,2号峰是瓜蒌的基本药效物质群;6,12,32,10,14,18,30,28,8,4,26,9号峰是瓜蒌条的基本药效物质群。6,14,12,32,30,4,26号峰为瓜蒌与瓜蒌条发挥药效的主要共有药效物质基础,其中6号峰为5-羟甲基糠醛,14号峰为香草酸,28号峰为芦丁,但与药效的关联度有所不同。瓜蒌及瓜蒌条抗大鼠心肌缺血-再灌注损伤作用来自与给药剂量有关的药效物质群间的协同作用。瓜蒌与瓜蒌条发挥药效的基本药效物质群不同,但存在共有有效成分群。
To investigate the spectrum-activity relationship of Trichosanthis Fructus and Trichosanthis Fructus strip pieces for rat myocardial ischemia-reperfusion injury.HPLC fingerprints of Trichosanthis Fructus and Trichosanthis Fructus strip pieces were established,and the values of creatinekinase-MB(CK-MB),myoglobin(MYO) and cardiac troponin-T(cTNT) in 3 dose groups(2.25,13.5,27.0 g·kg~(-1),equivalent to the crude herb g·kg~(-1)) of Trichosanthis Fructus and Trichosanthis Fructus strip pieces with myocardial ischemia-reperfusion injury in rats were measured,and the grey relational analysis was used to study the spectrum-activity relationship of Trichosanthis Fructus and Trichosanthis Fructus strip pieces for rat myocardial ischemia-reperfusion injury.With the dosage increase from 2.25 g·kg~(-1) to 27.0 g·kg~(-1),the correlation degree of spectrum-activity relationship of Trichosanthis Fructus and Trichosanthis Fructus strip pieces was also enhanced,but the change trend was different between these two groups.According to the frequency of the top 10 peaks in the correlation degree,peak 17,14,16,19,32,12,26,30,4,6 and 2 were the basic effective substances group of Trichosanthis Fructus,peak 6,14,12,32,30,4 and 6 were the basic effective substances group of Trichosanthis Fructus strip pieces.Peak 6,14,12,32,30,4 and 26 in fingerprints of Trichosanthis Fructus and Trichosanthis Fructus strip pieces were the main common pharmacodynamic substance base,among them,peak 6 was 5-hydroxymethyl furfural,peak 14 was vanillic acid and the peak28 was rutin,but the correlation degree with the efficacy was different.The effect of Trichosanthis Fructus and Trichosanthis Fructus strip pieces on rat myocardial ischemia-reperfusion injury was due to the synergistic effect of the effective substance groups related to the dosage.The essential pharmacodynamic substance groups of Trichosanthis Fructus and Trichosanthis Fructus strip pieces were different,but they shared a common active ingredient group.

关键词(KeyWords): 瓜蒌;瓜蒌条;指纹图谱;抗心肌缺血-再灌注损伤作用;谱效关系
Trichosanthis Fructus;Trichosanthis Fructus strip pieces;fingerprint spectrum;anti-myocardial ischemia-reperfusion injury;spectrum-activity relationship

Abstract:

Keywords:

基金项目(Foundation): 安徽高校省级自然科学研究重大项目(KJ2015ZD41,KJ2016SD60)

作者(Author): 邹纯才;宗倩妮;鄢海燕;
ZOU Chun-cai;ZONG Qian-ni;YAN Hai-yan;Pharmacy School of Wannan Medical College;

Email:

DOI: 10.19540/j.cnki.cjcmm.2018.0004

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