廖钦洪;邹勇;李洪雷;姜玉松;
LIAO Qin-hong;ZOU Youg;LI Hong-lei;JIANG Yu-song;Research Institute for Special Plants,Chongqing University of Arts and Sciences;Collabrative Innovation Center for Special Plant Industry of Chongqing;

• 1:重庆文理学院特色植物研究院
• 2:重庆市特色植物产业协同创新中心

摘要(Abstract)：

NAC家族是植物特有的转录因子,广泛参与植物生长发育、信号传导和逆境应答等过程。该研究基于生姜RNA-seq数据,利用生物信息学方法对生姜NAC家族成员、亚族分类、理化性质、蛋白质结构和表达模式进行预测和分析,结果显示:在271.1 Mb生姜转录组数据中筛选得到72个生姜NAC转录因子,通过进化树分析将其分为13个亚族;不同亚族NAC蛋白质的理化性质,如氨基酸数目、等电点等存在差异,二级结构主要以无规则卷曲为主要构成元件,三级结构相似性较高;23个NAC转录因子在不同土壤湿度及青枯菌Ralstonia solanacearum侵染处理下呈现显著的表达差异,其主要分布在与植物衰老、激素代谢和细胞壁代谢相关的第Ⅻ,ⅩⅤ,Ⅷ亚族。该研究结果为生姜NAC基因家族的研究和利用提供了理论依据。
The NAC family is an important transcription factor which regulate plant growth and development,signal transduction,and stress response. In this study,the protein identification,subfamily classification,the determination of physical and chemical properties,protein structure,and expression pattern of NAC family were performed using bioinformatic methods based on the RNA-seq data of ginger. The results showed that a total of 72 NAC transcription factors were identified in 271. 1 Mb total nucleotides,and they could be clustered into 13 subfamilies according to the phylogenetic tree. The physical and chemical properties,structure analysis revealed that the amino acid number and isoelectric point were different among 13 NAC subfamilies; the secondary structure of NACs transcription factors mainly consist of random coil,and the tertiary structure is similar. In addition,the expression patterns of genes under different soil moisture and Ralstonia solanacearum infection showed that 23 NACs were differentially expressed,which were mainly distributed in Ⅷ,Ⅶ,and Ⅹ Ⅴ subfamilies related to plant senescence,hormone metabolism and cell wall metabolism. The results provide some valuable information for the research and development of NAC transcription factors in ginger.

关键词(KeyWords)： 生姜;NAC转录因子;RNA-seq;基因差异表达
ginger;NAC transcription factor;RNA-seq;gene differential expression

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学青年基金项目(31501273);; 重庆市前沿与应用基础研究项目(cstc2015jcyj A8003)

作者(Authors): 廖钦洪;邹勇;李洪雷;姜玉松;
LIAO Qin-hong;ZOU Youg;LI Hong-lei;JIANG Yu-song;Research Institute for Special Plants,Chongqing University of Arts and Sciences;Collabrative Innovation Center for Special Plant Industry of Chongqing;

DOI: 10.19540/j.cnki.cjcmm.20180105.007

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