廖钦洪;邹勇;李洪雷;姜玉松;
LIAO Qin-hong;ZOU Youg;LI Hong-lei;JIANG Yu-song;Research Institute for Special Plants,Chongqing University of Arts and Sciences;Collabrative Innovation Center for Special Plant Industry of Chongqing;

• 1:重庆文理学院特色植物研究院
• 2:重庆市特色植物产业协同创新中心

摘要(Abstract)：

NAC家族是植物特有的转录因子,广泛参与植物生长发育、信号传导和逆境应答等过程。该研究基于生姜RNA-seq数据,利用生物信息学方法对生姜NAC家族成员、亚族分类、理化性质、蛋白质结构和表达模式进行预测和分析,结果显示:在271.1 Mb生姜转录组数据中筛选得到72个生姜NAC转录因子,通过进化树分析将其分为13个亚族;不同亚族NAC蛋白质的理化性质,如氨基酸数目、等电点等存在差异,二级结构主要以无规则卷曲为主要构成元件,三级结构相似性较高;23个NAC转录因子在不同土壤湿度及青枯菌Ralstonia solanacearum侵染处理下呈现显著的表达差异,其主要分布在与植物衰老、激素代谢和细胞壁代谢相关的第Ⅻ,ⅩⅤ,Ⅷ亚族。该研究结果为生姜NAC基因家族的研究和利用提供了理论依据。
The NAC family is an important transcription factor which regulate plant growth and development,signal transduction,and stress response. In this study,the protein identification,subfamily classification,the determination of physical and chemical properties,protein structure,and expression pattern of NAC family were performed using bioinformatic methods based on the RNA-seq data of ginger. The results showed that a total of 72 NAC transcription factors were identified in 271. 1 Mb total nucleotides,and they could be clustered into 13 subfamilies according to the phylogenetic tree. The physical and chemical properties,structure analysis revealed that the amino acid number and isoelectric point were different among 13 NAC subfamilies; the secondary structure of NACs transcription factors mainly consist of random coil,and the tertiary structure is similar. In addition,the expression patterns of genes under different soil moisture and Ralstonia solanacearum infection showed that 23 NACs were differentially expressed,which were mainly distributed in Ⅷ,Ⅶ,and Ⅹ Ⅴ subfamilies related to plant senescence,hormone metabolism and cell wall metabolism. The results provide some valuable information for the research and development of NAC transcription factors in ginger.

关键词(KeyWords)： 生姜;NAC转录因子;RNA-seq;基因差异表达
ginger;NAC transcription factor;RNA-seq;gene differential expression

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学青年基金项目(31501273);; 重庆市前沿与应用基础研究项目(cstc2015jcyj A8003)

作者(Author): 廖钦洪;邹勇;李洪雷;姜玉松;
LIAO Qin-hong;ZOU Youg;LI Hong-lei;JIANG Yu-song;Research Institute for Special Plants,Chongqing University of Arts and Sciences;Collabrative Innovation Center for Special Plant Industry of Chongqing;

Email:

DOI: 10.19540/j.cnki.cjcmm.20180105.007

参考文献(References)：

• [1]靳进朴,郭安源,何坤,等.植物转录因子分类、预测和数据库构建[J].生物技术通报,2015,31(11):68.
• [2]Vroemen C W,Mordhorst A P,Albrecht C,et al.The cupshaped cotyledon3 gene is required for boundary and shoot meristem formation in Arabidopsis[J].Plant Cell,2003,15(7):1563.
• [3]Aida M,Ishida T,Fukaki H,et al.Genes involved in organ separation in Arabidopsis:an analysis of the cup-shaped cotyledon mutant[J].Plant Cell,1997,9(6):841.
• [4]Ko J H,Yang S H,Park A H,et al.ANAC012,a member of the plant-specific NAC transcription factor family,negatively regulates xylary fiber development in Arabidopsis thaliana[J].Plant J,2007,50(6):1035.
• [5]Zhong R,Lee C,Ye Z H.Global analysis of direct targets of secondary wall NAC master switches in Arabidopsis[J].Mol Plant,2010,3(6):1087.
• [6]He X J,Mu R L,Cao W H,et al.At NAC2,a transcription factor downstream of ethylene and auxin signaling pathways,is involved in salt stress response and lateral root development[J].Plant J,2005,44(6):903.
• [7]Kikuchi K,Ueguchi-Tanaka M,Yoshida K T,et al.Molecular analysis of the NAC gene family in rice[J].Mol Gen Genet,2000,262(6):1047.
• [8]Nakashima K,Tran L S P,Van Nguyen D,et al.Functional analysis of a NAC-type transcription factor Os NAC6 involved in abiotic and biotic stress-responsive gene expression in rice[J].Plant J,2007,51(4):617.
• [9]Wang X,Basnayake B M V S,Zhang H,et al.The Arabidopsis ATAF1,a NAC transcription factor,is a negative regulator of defense responses against necrotrophic fungal and bacterial pathogens[J].Mol Plant Microbe In,2009,22(10):1227.
• [10]Delessert C,Kazan K,Wilson I W,et al.The transcription factor ATAF2 represses the expression of pathogenesis-related genes in Arabidopsis[J].Plant J,2005,43(5):745.
• [11]Kamiuchi Y,Yamamoto K,Furutani M,et al.The CUC1 and CUC2 genes promote carpel margin meristem formation during Arabidopsis gynoecium development[J].Front Plant Sci,2014,30(5):165.
• [12]Feng H,Duan X,Zhang Q,et al.The target gene of taemiR164,a novel NAC transcription factor from the NAM subfamily,negatively regulates resistance of wheat to stripe rust[J].Mol Plant Pathol,2014,15(3):284.
• [13]Ramaswamy M,Narayanan J,Manickavachagam G,et al.Genome wide analysis of NAC gene family'sequences'in sugarcane and its comparative phylogenetic relationship with rice,sorghum,maize and Arabidopsis for prediction of stress associated NAC genes[J].Agri Gene,2017,3:1.
• [14]方文韬,詹志来,彭华胜,等.干姜,生姜,炮姜分化的历史沿革与变迁[J].中国中药杂志,2017,42(9):1641.
• [15]姚磊,张宁,杨江伟,等.马铃薯miRNA164靶基因预测及amiRNA164表达载体构建[J].分子植物育种,2016,14(6):1482.
• [16]孙欣,上官凌飞,房经贵,等.葡萄NAC转录因子家族生物信息学分析[J].基因组学与应用生物学,2011,30(2):229.
• [17]李伟,韩蕾,钱永强,等.植物NAC转录因子的种类、特征及功能[J].应用与环境生物学报,2011,17(4):596.
• [18]陈秀玲,王傲雪,张珍珠,等.番茄NAC转录因子家族的鉴定及生物信息学分析[J].植物生理学报,2014,50(4):461.
• [19]王洋,柏锡.大豆NAC基因家族生物信息学分析[J].大豆科学,2014,33(3):325.
• [20]葛姗姗,唐桂英,毕玉平,等.玉米全基因组中NAC基因家族的鉴定与分析[J].山东农业科学,2015,47(2):1.
• [21]韩雅彭,程琳,杨凌霄,等.茶树NAC转录因子家族的鉴定及生物信息学分析[J].河南大学学报,2017,47(3):301.
• [22]黎帮勇,胡尚连,曹颖,等.毛竹NAC转录因子家族生物信息学分析[J].基因组学与应用生物学,2015,34(8):1769.
• [24]Sun L,Zhang H,Li D,et al.Functions of rice NAC transcriptional factors,ONAC122 and ONAC131,in defense responses against Magnaporthe grisea[J].Plant Mol Biol,2013,81(1/2):41.
• [25]Rauf M,Arif M,Fisahn J,et al.NAC transcription factor speedy hyponastic growth regulates flooding-induced leaf movement in Arabidopsis[J].Plant Cell,2013,25(12):4941.
• [26]Kunieda T,Mitsuda N,Ohme-Takagi M,et al.NAC family proteins NARS1/NAC2 and NARS2/NAM in the outer integument regulate embryogenesis in Arabidopsis[J].Plant Cell,2008,20:2631.