中国中药杂志

2018, v.43(03) 478-483

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内生细菌BZJN1的鉴定及对白术根腐病的生物防治研究
Identification of endophytic bacteria BZJN1 and research on biological control of root rot of Atractylodes macrocephala

游景茂;熊坤;穆森;郭杰;郭晓亮;段媛媛;李娟;曹凡;邹宗成;谭海;
YOU Jing-mao;XIONG Kun;MU Sen;GUO Jie;GUO Xiao-liang;DUAN Yuan-yuan;LI Juan;CAO Fan;ZOU Zong-cheng;TAN Hai;Institute of Chinese Herbal Medicines,Hubei Academy of Agricultural Sciences;Enshi for Food and Drug Inspection and Testing Center;Ba Dong Jin Da Pharmaceutical Co.,Ltd.;

摘要(Abstract):

该研究从白术体内分离得到一株细菌BZJN1,经生理生化检测及分子鉴定证明其为枯草芽孢杆菌Bacillus subtilis。菌株BZJN1可以显著抑制白术角担菌菌丝生长,抑制率超过70%。显微观察对峙区域发现角担菌菌丝生长畸形、菌丝顶端或中部膨大呈球形,原生质泄露。其发酵滤液在3%,6%的浓度下对角担菌菌丝生长抑制率达到22.7%,38.7%。田间防效试验证明,药后10 d,BZJN1生防菌剂在1×10~9cfu·mL~(-1)处理防效最高,达到了75.27%。药后20 d,1×10~9cfu·mL~(-1)的处理防效为72.37%,和化学药剂相当。所有BZJN1的处理对白术有一定促生效果,1×10~9cfu·mL~(-1)处理,增产达到14.1%。
In this study,an endophytic bacteria strain BZJN1 was isolated from Atractylodes macrocephala,and identified as Bacillus subtilis by physiological and biochemical tests and molecular identification. Strain BZJN1 could inhibit the growth of mycelia of Ceratobasidium sp. significantly,and the inhibition rate was more than 70%. The mycelium growth deformity with bulge as spherical and partially exhaustible in apex or central with microscopic observation. The inhibitory rates under 3% and 6% concentrations of the cell free fermentation were 22. 7% and 38. 7% expectively. The field test proved that the control efficacy of treatment of 1 × 10~9cfu·mL~(-1) is 75. 27% and 72. 37% after 10 and 20 days. All the treatments of strain BZJN1 was able to promote the growth of A. macrocephala,the treatment of 1 × 10~9cfu·mL~(-1) could able to increase the yield to 14. 1%.

关键词(KeyWords): 枯草芽孢杆菌;白术;角担菌;防治
Bacillus subtilis;Atractylodes macrocephala;Ceratobasidium sp;control

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基金项目(Foundation): 湖北省自然科学基金面上项目(2016CFB521);; 湖北省农业科学院竞争性项目(2016JZXJH019);湖北省农业科学院青年科学基金项目(2018NKYJJ16);; 国家现代农业产业技术体系建设专项(CARS-21);; 湖北省技术创新专项(民族专项MZZX2018000005)

作者(Author): 游景茂;熊坤;穆森;郭杰;郭晓亮;段媛媛;李娟;曹凡;邹宗成;谭海;
YOU Jing-mao;XIONG Kun;MU Sen;GUO Jie;GUO Xiao-liang;DUAN Yuan-yuan;LI Juan;CAO Fan;ZOU Zong-cheng;TAN Hai;Institute of Chinese Herbal Medicines,Hubei Academy of Agricultural Sciences;Enshi for Food and Drug Inspection and Testing Center;Ba Dong Jin Da Pharmaceutical Co.,Ltd.;

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