中国中药杂志

2019, v.44(24) 5433-5440

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不同采收期祁艾化学成分差异性研究
Study on difference of chemical constituents of Qiai in different harvest periods

薛紫鲸;郭利霄;郭梅;杨贵雅;张丹;郭龙;郑玉光;
XUE Zi-jing;GUO Li-xiao;GUO Mei;YANG Gui-ya;ZHANG Dan;GUO Long;ZHENG Yu-guang;College of Pharmacy,Hebei University of Chinese Medicine;

摘要(Abstract):

艾叶为菊科植物艾Artemisia argyi的干燥叶,祁艾为艾叶道地药材之一。该研究为了明确不同采收期祁艾药材中化学成分的动态变化规律,为祁艾药材适时采收期确定提供科学依据,采用紫外-可见分光光度法对6个采收时期共36批祁艾样品总黄酮和总酚酸含量进行了测定,进一步采用高效液相色谱法对其祁艾样品中8个指标性成分,包括6个酚酸类成分(新绿原酸、绿原酸、隐绿原酸、异绿原酸A、异绿原酸B、异绿原酸C)和2个黄酮类成分(棕矢车菊素、异泽兰黄素)进行了含量测定。依据含量测定结果,从总黄酮、总酚酸、指标性成分等方面分析了不同采收期祁艾化学成分差异及动态变化,结果表明不同采收期祁艾化学成分含量存在一定的差异。不同采收期祁艾总黄酮和总酚酸含量变化规律较为一致,均以第3个采收时期(端午节前后)的含量最高;黄酮类指标成分含量变化趋势与总黄酮含量变化趋势较为一致,而酚酸类指标成分的含量变化趋势与总酚酸变化趋势不一致。通过以上研究,为祁艾药材质量评价和最佳采收期的确定提供了分析方法和数据支撑。
Artemisiae Argyi Folium,the dried leaves of Artemisia argyi,has been widely used in traditional Chinese and folk medicines for a long time. Qiai is one of the top-geoherb of Artemisiae Argyi Folium. Trying to investigate dynamic changes of chemical components of Qiai in different harvest periods and explore the optimum harvest time of Qiai,in this study,the contents of total flavonoids and total phenolic acids of 36 batches of Qiai collected in 6 different harvest periods were analyzed by ultraviolet-visible spectrophotometry. Furthermore,an HPLC method was applied for simultaneous determination of eight bioactive compounds including six phenolic acids( 5-caffeoylquinic acid,3-caffeoylquinic acid,4-caffeoylquinic acid,3,4-di-O-caffeoylquinic acid,3,5-di-O-caffeoylquinic acid and 4,5-di-O-caffeoylquinic acid) and two flavonoids( jaceosidin and eupatilin) in Qiai samples. The quantitative results indicated that there were some differences in the contents of total flavonoids,total phenolic acids and bioactive compounds of Qiai samples in different harvest periods. The dynamic changes of total flavonoids and total phenolic acids of Qiai in different harvest periods were consistent. The contents of total flavonoids and total phenolic acids of Qiai samples were higher in the third harvest period( around the Dragon Boat Festival),which is basically consistent with the traditional harvest periods. This present study can provide the basis for determining the suitable harvest time of Qiai,and might be useful for the quality evaluation of this herbal medicine.

关键词(KeyWords): 祁艾;采收期;黄酮;酚酸;含量测定
Qiai;harvest periods;flavonoids;phenolic acids;quantitative analysis

Abstract:

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基金项目(Foundation): 河北省自然科学基金项目(H2018423032);; 河北省普通高等学校青年拔尖人才计划项目(BJ2019006);; 孙宝惠全国名老中医药专家传承工作室(7002016008005);; 河北省二期现代农业产业技术体系创新团队项目(HBCT2018060205);; 河北省中医药管理局科研计划项目(2018108);; 第四次全国中药资源普查(Z135080000022)

作者(Author): 薛紫鲸;郭利霄;郭梅;杨贵雅;张丹;郭龙;郑玉光;
XUE Zi-jing;GUO Li-xiao;GUO Mei;YANG Gui-ya;ZHANG Dan;GUO Long;ZHENG Yu-guang;College of Pharmacy,Hebei University of Chinese Medicine;

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