中国中药杂志

2008, (09) 1049-1052

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天麻素对大鼠皮层缺氧神经细胞NR1亚基mRNA表达的影响
Expression of NR1 mRNA of NMDA receptor by Gastrodine on hypoxia injury in cultured rat cerebral cortical neurons

付蕾;毛艳华;高远;刘蕾;王志平;李良辰;
FU Lei1,MAO Yan-hua1,GAO Yuan2,LIU Lei1,WANG Zhi-ping1,LI Liang-chen2 (1.Pharmacy College,Zengzhou University,Zhengzhou 450001,China; 2.Basic Medical College of Zhengzhou University,Zhengzhou 450001,China)

摘要(Abstract):

目的:观察天麻素对体外培养大鼠皮层缺氧神经细胞N-甲基-D-天冬氨酸(NMDA)受体NR1亚基mRNA表达的影响,探讨天麻素在神经保护方面的可能作用机制。方法:体外培养大鼠胚胎皮层神经细胞,以缺氧无糖培养建立神经细胞损伤模型,采用不同质量浓度的天麻素(13,26,52 mg.L-1)处理细胞,用反转录聚合酶链式反应(RT-PCR)法检测神经细胞NR1 mRNA的表达。结果:神经细胞缺氧损伤后,NR1 mRNA的表达显著增高;加入不同质量浓度的天麻素后均能显著降低神经细胞NR1 mRNA的表达。其中以26,52 mg.L-1的抑制作用最佳。结论:天麻素可能通过拮抗由缺氧引起的神经细胞NMDA受体NR1亚基mRNA表达的增加,发挥其神经保护作用。
Objective: To investigate the effect of gastrodine on the expression of NR1 mRNA of NMDA receptor in cultured rat cerebral cortical neurons injury induced by hypoxia.Method: The injury models of cultured rat cerebral cortical neurons was made by hypoxia and hypoglucose.The concentration of 13,26,52 mg·L-1 of gastrodine was respectively added to the injured cells.The R-PCR technique was used to study the expression of NR1 mRNA of NMDA receptor.Result: The expression of NR1 mRNA increased markedly in hypoxia and hypoglucose injured cells,the increased expression can be weakened by gastrodine,more significant effect was found in the concentration of 26 mg·L-1和 52 mg·L-1.Conclusion: Gastrodine may act as a neuro-protector by weakening the expression of NR1 mRNA in cultured rat cerebral cortical neurons injury induced by hypoxia and hypoglucose.

关键词(KeyWords): 天麻素;神经细胞;缺氧;NR1mRNA;反转录聚合酶链式反应
gastrodine;cortical neurons;hypoxia;NR1 mRNA;RT-PCR

Abstract:

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基金项目(Foundation): 河南省医学科技攻关项目(20050014)

作者(Author): 付蕾;毛艳华;高远;刘蕾;王志平;李良辰;
FU Lei1,MAO Yan-hua1,GAO Yuan2,LIU Lei1,WANG Zhi-ping1,LI Liang-chen2 (1.Pharmacy College,Zengzhou University,Zhengzhou 450001,China; 2.Basic Medical College of Zhengzhou University,Zhengzhou 450001,China)

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