中国中药杂志

2015, v.40(02) 275-279

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HPLC指纹图谱鉴别葶苈子与车前子
Discrimination of Descurainiae Semen and Pantagirus Semen by HPLC fingerprints

周喜丹;唐力英;周国洪;寇真真;王婷;王祝举;
ZHOU Xi-dan;TANG Li-ying;ZHOU Guo-hong;KOU Zhen-zhen;WANG Ting;WANG Zhu-ju;Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences;

摘要(Abstract):

目的:鉴别葶苈子与车前子。方法:采用高效液相色谱法建立南葶苈子指纹图谱,并使用相似度评价软件,系统聚类分析和偏最小二乘法判别分析对两者的HPLC图谱进行识别研究。结果:葶苈子与车前子的HPLC色谱峰和相对峰面积存在较大的差异,经统计分析,所有样品被分为葶苈子、车前子和混淆品三类。结论:HPLC指纹图谱结合系统聚类分析和偏最小二乘法判别分析等化学计量学方法可用来鉴别葶苈子和车前子,该方法快速、简便、准确、有效,可为葶苈子和车前子的质量评价提供依据。
Objective: To discriminate Descurainiae Semen and Pantagirus Semen. Method: A high-performance liquid chromatographic method was developed to establish the fingerprint of Descurainiae Semen,and hierarchical cluster analysis( HCA) and partial least squares discriminant analysis( PLS-DA) were applied to study HPLC fingerprinting and chemical recognition. Result: There exists large difference of chromatographic peaks and its relative peak area of HPLC fingerprints between Descurainiae Semen and Pantagirus Semen,and after conducting statistical analysis,the result demonstrated that all samples were classified into three categories: Descurainiae Semen,Pantagirus Semen and their mixtures. Conclusion: The developed HPLC fingerprint combined with chemometrics can be utilized to discriminate between Descurainiae Semen and Pantagirus Semen,which was quick,simple,accurate and reliable and can provide the basis for the characterization and quality assess of Descurainiae Semen and Pantagirus Semen.

关键词(KeyWords): 南葶苈子;平车前子;指纹图谱;化学计量学
Descurainiae Semen;Pantagirus Semen;HPLC fingerprints;chemometrics

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作者(Author): 周喜丹;唐力英;周国洪;寇真真;王婷;王祝举;
ZHOU Xi-dan;TANG Li-ying;ZHOU Guo-hong;KOU Zhen-zhen;WANG Ting;WANG Zhu-ju;Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences;

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