中国中药杂志

2013, v.38(23) 4113-4118

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紫皮石斛的质量标准研究
Studies on quality evaluation of Dendrobii devoniani

甘小娜;徐英;徐红;刘家保;杨莉;王峥涛;
GAN Xiao-na;XU Ying;XU Hong;LIU Jia-bao;YANG Li;WANG Zheng-tao;The MOE Key Laboratory for Standardization of Chinese Medicines and Key Laboratory of Complex Prescription, Institute of Chinese Materia Medica,Shanghai University of Traditional Chinese Medicine;Shanghai R&D Center for Standardization of Traditional Chinese Medicines;Forestry Administration of Longling County;

摘要(Abstract):

为建立云南省龙陵县紫皮石斛的地方质量标准,采用硫酸-苯酚法测定紫皮石斛中多糖的含量;采用高效液相色谱法测定药材中甘露糖的含量,并根据2010年版《中国药典》相关附录测定浸出物。结果表明紫皮石斛的显微鉴别特征性强;薄层色谱分离度好,斑点清晰;紫皮石斛含多糖质量分数为35.7%~52.1%(平均42.7%),甘露糖为27.8%~46.1%(平均35.8%),浸出物为4.5%~10.6%(平均7.38%)。所建方法操作简单,准确可靠,重复性好,制定的标准限度合理,可用于评价云南省龙陵县紫皮石斛的质量。
To establish the local quality standard for Dendrobii devoniani caulis from Longling,Yunnan,the pharmacognostic characteristics microscopic characteristics and TLC identification were developed. Sulfuric acid-phenol method was used to determine the content of polysaccharide. An HPLC method was adopted to determine the content of mannose,and extractives were determined according to the procedures recorded in the Appendix of Chinese Pharmacopoeia( 2010). The results showed a strong characteristics microscopic of Dendrobii devoniani caulis,and its TLC identification had a good resolution with clear spots; the content of polysaccharide is 35. 7%-52. 1%( average 42. 7%),mannose 27. 8%-46. 1%( average 35. 8%),and extract 4. 5%-10. 6%( average 7. 38%). The method is simple,accurate and reliable,with good reproducibility. The established standard is acceptable for quality evaluation of Dendrobii devoniani caulis from Longling,Yunnan.

关键词(KeyWords): 紫皮石斛;质量标准;显微鉴别;薄层鉴别;含量测定
Dendrobii devoniani;quality standard;TLC identification;assay

Abstract:

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基金项目(Foundation): 国家自然科学基金优秀青年基金项目(81222053)

作者(Author): 甘小娜;徐英;徐红;刘家保;杨莉;王峥涛;
GAN Xiao-na;XU Ying;XU Hong;LIU Jia-bao;YANG Li;WANG Zheng-tao;The MOE Key Laboratory for Standardization of Chinese Medicines and Key Laboratory of Complex Prescription, Institute of Chinese Materia Medica,Shanghai University of Traditional Chinese Medicine;Shanghai R&D Center for Standardization of Traditional Chinese Medicines;Forestry Administration of Longling County;

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