中国中药杂志

2015, v.40(12) 2378-2382

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西红花苷对照提取物的研究及其在西红花饮片质量控制中的应用
Study on crocins reference extract and application on assay of Croci Stigma

何风艳;戴忠;何轶;张聿梅;鲁静;
HE Feng-yan;DAI Zhong;HE Yi;ZHANG Yu-mei;LU Jing;National Institutes for Food and Drug Control;

摘要(Abstract):

为了解决色谱分析中西红花苷Ⅰ,Ⅱ对照品稳定性差、价格较高的问题,提出采用对照提取物进行质量控制的方法。以廉价易得的栀子黄色素为原料制备西红花苷对照提取物,采用单体对照品标定其中西红花苷Ⅰ,Ⅱ的含量,并对提取物的稳定性影响因素进行考察。采用对照提取物对12批西红花饮片进行分析,结果与采用单体对照品测得的结果基本一致。该方法可用于西红花饮片的质量控制,初步验证了对照提取物替代单体对照品用于中药质量控制的可行性。
High price and poor stability of both crocin-1 and crocin-2 reference substance have become obstacles to HPLC assay of Croci Stigma. A new method based on reference extract was proposed. In this study,the reference extract was prepared from gardenia yellow which is cheap and easy to get. The content of crocin-1 and crocin-2 in reference extract was determined and factors affecting stability of reference extract were investigated. Twelve batches of Croci Stigma were analyzed with reference extract and reference substance respectively. The results showed no difference. The presented method is feasible for quality control of Croci Stigma and reference extract is suitable to replace reference substances in assay.

关键词(KeyWords): 对照提取物;西红花;质量控制;栀子黄色素
reference extract;Croci Stigma;quality control;gardenia yellow

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基金项目(Foundation): 中国食品药品检定研究院中青年发展研究基金项目(2012A9)

作者(Author): 何风艳;戴忠;何轶;张聿梅;鲁静;
HE Feng-yan;DAI Zhong;HE Yi;ZHANG Yu-mei;LU Jing;National Institutes for Food and Drug Control;

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