中国中药杂志

2020, v.45(03) 572-578

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经典名方散偏汤HPLC指纹图谱的建立及川芎的量值传递研究
HPLC fingerprint of famous traditional formula Sanpian Decoction and quality value transmitting of Chuanxiong Rhizoma

莫雨佳;王彦;齐琪;喻祥龙;罗菊元;胡海燕;刘峰;吴建雄;陆洋;杜守颖;白洁;李鹏跃;
MO Yu-jia;WANG Yan;QI Qi;YU Xiang-long;LUO Ju-yuan;HU Hai-yan;LIU Feng;WU Jian-xiong;LU Yang;DU Shou-ying;BAI Jie;LI Peng-yue;Beijing University of Chinese Medicine;Yifan Pharmaceutical Co., Ltd.;

摘要(Abstract):

经典名方散偏汤出自清朝医家陈士铎的《辩证录》,隶属《古代经典名方目录(第一批)》100个经典名方之中。参照《医疗机构中药煎药室管理规范》,制备散偏汤的水煎液。根据散偏汤中不同成分的极性大小,建立2张HPLC图,其中川芎、白芍、白芥子、甘草、郁李仁、白芷6味药在指纹图谱中均有体现;以出膏率、转移率、指纹图谱相似度为指标,研究处方中川芎药材-饮片-全方水煎液的量值传递关系。结果显示,川芎中指标性成分阿魏酸从药材到饮片的转移率72.00%~108.36%,饮片到全方的阿魏酸转移率31.76%~64.09%;全方的出膏率14.69%~20.16%;15批全方指纹图谱1相似度0.971~0.998,指纹图谱2相似度0.980~0.996。该文建立的指纹图谱信息丰富,所建立的质量评价方法适用于川芎药材-饮片-全方水煎液质量控制,可以为配方颗粒、经典名方及其他源于中药水煎液的终端产品质量控制评价办法的制定,提供一定的参考。
Famous traditional formula Sanpian Decoction(SPD)comes from Dialectical Records of Chen Shiduo of the Qing Dynasty,and ranks among 100 classic prescriptions of Classic Famous Traditional Formula catalogue(the First Batch). SPD was prepared according to Management Standards for Traditional Chinese Medicine Decoction Room in Medical Institutions. According to the polarity of different components in SPD,two HPLC fingerprints were established, in which six herbs, namely Chuanxiong Rhizoma, Paeoniae Randix Alba, Sinapis Semen, Glycyrrhizae Radix et Rhizoma, Pruni Semen, Angelicae Dahuricae Radix,are all reflected in the fingerprints; The dry extract rate, transfer rate and similarities of fingerprints were used as indicators to study the relationship between the quality value transmitting of medicinal herbs-decoction pieces-whole decoction of Chuanxiong Rhizoma. Experiment result shows that,the transfer rate of ferulic acid from medicinal herbs to decoction pieces is between 72.00% and 108.36%; the transfer rate of ferulic acid from decoction pieces to SPD is between 31.76% and 64.09%; the dry extract rate of the whole decoction is between 14.69% and 20.16%;The similarity range of fingerprint 1 of 15 batches of SPD is between 0.971 and 0.998, and the similarity range of fingerprint 2 is between 0.980 and 0.996. The established fingerprint has rich information,and the established quality evaluation method is suitable for the quality control of medicinal herbs-decoction pieces-whole decoction of Chuanxiong Rhizoma, which can provide a certain reference for developing the quality control evaluation method for formulated granules, famous formulae and other terminal products derived from traditional Chinese medicine decoction.

关键词(KeyWords): 经典名方;散偏汤;水煎液;川芎;指纹图谱;量值传递
famous traditional formula;Sanpian Decoction;decoction;Chuanxiong Rhizoma;fingerprint;quality value transmitting

Abstract:

Keywords:

基金项目(Foundation): 2019年北京中医药大学校级课题项目(1000061222557)

作者(Author): 莫雨佳;王彦;齐琪;喻祥龙;罗菊元;胡海燕;刘峰;吴建雄;陆洋;杜守颖;白洁;李鹏跃;
MO Yu-jia;WANG Yan;QI Qi;YU Xiang-long;LUO Ju-yuan;HU Hai-yan;LIU Feng;WU Jian-xiong;LU Yang;DU Shou-ying;BAI Jie;LI Peng-yue;Beijing University of Chinese Medicine;Yifan Pharmaceutical Co., Ltd.;

Email:

DOI: 10.19540/j.cnki.cjcmm.20191001.303

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