中国中药杂志

1996, (09)

[打印本页] [关闭]
本期目录(Current Issue) | 过刊浏览(Past Issue) | 高级检索(Advanced Search)

HPLC法分离制备斑衣蜡蝉中的吲哚类生物碱
SeparationandPreparationofIndoleAlkaloidsinLycormadelicatulaWhite.byHPLC

薛公达,原思通
XueGongdaandYuanSitong(InstituteofChineseMateriaMedica,ChinaAcademyofTraditionalChineseMedicine,Beijing100700)

摘要(Abstract):

采用BIO-RADHi-PoreRP318柱,以80%甲醇-水(梯度设置)为流动相,检测波长为254nm,首次从传统昆虫类中药樗鸡(学名斑衣蜡蝉)中分离并制备了4种生物碱。经化学和光谱鉴定,确定了其中2种吲哚类生物碱β-yohimbine和ajmalicine。
AHPLCmethodforseparatingandpreparingindolealkaloidsisdescribed.HPLCconditionsforana-lysis:BIO-RADseries700HPLC,model700datastation,UV:model1740UV-VISmonitor,column:BIO-RADHi-poreRP318,250mm×10mm,mobilephase:80%methanol-H2O(gradient),flowrate:1.5ml/min,dc-tectionwavelength:254nm.Onthebasisofspectral(1HNMR,13CNMR,H-HCOSY,MS,DEPT)andchemicalevidence,thestructuresoftwocompoundswereelucidatedasβ-yohimbine(yohimban-16-carboxylicacid-17-hy-droxymethylester(3α,16α,17β))andajmalicine(oxayohimban-16-carboxylicacid-16,17-didehydro-19-ethylmethylester(19α)).

关键词(KeyWords): 斑衣蜡蝉,HPLC,吲哚类生物碱
Lycormadelicatula;HPLC;indolealkaloid

Abstract:

Keywords:

基金项目(Foundation):

作者(Author): 薛公达,原思通
XueGongdaandYuanSitong(InstituteofChineseMateriaMedica,ChinaAcademyofTraditionalChineseMedicine,Beijing100700)

扩展功能
本文信息
服务与反馈
本文关键词相关文章
本文作者相关文章
中国知网
分享