中国中药杂志

2004, (10)

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红花注射液对大鼠局灶性脑缺血后梗死体积和神经元凋亡相关蛋白bcl-2,caspase-3表达的影响
Effects of Carthamus tinctorius injection on bcl-2,caspase-3 expression related to neurons apoptosis after local cerebral ischemia

罗嘉,方治平,周黎明,来松涛
LUO Jia 1, FANG Zhi-ping 1, ZHOU Li-ming 1, LAI Song-tao 2 (1.Pharmacology Department, West China Medical Center of Sichuan Un iversity, Chengdu 610041, China; 2.Emergency Center, Luoyang 471000, China) [

摘要(Abstract):

目的 :探讨红花注射液对神经元凋亡相关蛋白bcl 2 ,Caspase 3表达的影响。方法 :将SD大鼠随机分为缺血对照组、红花大、中、小剂量组 ,采用大鼠大脑中动脉栓塞模型以及TTC染色和免疫组织化学染色方法 ,在不同时间点观察各组大鼠脑梗死体积的变化以及bcl 2 ,Caspase 3阳性细胞表达的不同。结果 :在不同时间点各给药组大鼠的脑梗死体积低于缺血对照组 ,有统计学意义 (P <0 0 5 ) ;大剂量组脑梗死体积与中剂量组和小剂量组脑梗死体积相比亦有显著性差异 (P <0 0 5 ) ,而中、小剂量组之间比较无显著性差异 (P >0 0 5 )。各给药组大鼠bcl 2阳性细胞表达的数目均高于缺血对照组 ,而Caspase 3阳性细胞表达数目则低于缺血对照组 (P <0 0 5 ) ;大剂量组阳性细胞表达的数目与中剂量组和小剂量组阳性细胞表达的数目相比亦有显著性差异 (P <0 0 5 ) ,而中、小剂量组之间比较无显著性差异 (P >0 0 5 )。结论 :红花注射液可减少脑梗死体积 ,在脑缺血损伤急性期可增加bcl 2的表达而减少Caspase 3的表达
Objective: To investigate the effects of Carthamus tinctorius on bcl-2, caspase-3 expression of apoptosis of neurons . Method: SD rats were randomLy divided into ischemia control group, large-dose group, middle-dose group and low-dose group. The middle cerebral artery of rats was occluded for 2h by inserting an introluminal molofil ament, and reperfusion was then instituted for 4h or 22 h. The brains were sta ined with 2, 3, 5-triphenylte trazolinm chloride for assessment of volume of infarction, and then embedded onto slides with paraffin for morphological asse ssment and immunohistochemistry was carried out to investigate the changes in bc l-2 and caspase-3. Result: All treated groups at diff erent times decreased the volume of infarction (P<0.05), while large-dos e group showed more distinct decrease than other groups (P<0.05). All tre ated groups at different times increased bcl-2 and decreased caspase-3 express ion as well, while large-dose group showed more distinct effect (P<0.05) . Conclusion: C.tinctorius injection can reduce the volume of cerebral infarction, and increased bcl-2 and decreased caspase-3 e xpression. [

关键词(KeyWords): 脑梗死;红花;细胞凋亡;bcl2;Caspase3
cerebral ischemia injection; Carthamus t inctorius injection;apoptosis; bcl-2; caspase-3

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作者(Author): 罗嘉,方治平,周黎明,来松涛
LUO Jia 1, FANG Zhi-ping 1, ZHOU Li-ming 1, LAI Song-tao 2 (1.Pharmacology Department, West China Medical Center of Sichuan Un iversity, Chengdu 610041, China; 2.Emergency Center, Luoyang 471000, China) [

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