中国中药杂志

2020, v.45(18) 4398-4403

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炒王不留行标准汤剂质量标准研究
Study on quality standard of fried Vaccariae Semen standard decoction

王珊;刘博男;吕新林;杨娇艳;张永欣;梁曜华;史辑;朱晶晶;
WANG Shan;LIU Bo-nan;LYU Xin-lin;YANG Jiao-yan;ZHANG Yong-xin;LIANG Yao-hua;SHI Ji;ZHU Jing-jing;School of Pharmacy, Shaanxi Institute of Internal Trade & Commerce;Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences;School of Pharmacy, Liaoning University of Traditional Chinese Medicine;Anhui University of Chinese Medicine;

摘要(Abstract):

该研究以炒王不留行标准汤剂为研究对象,根据中药饮片标准汤剂的制备原则,制备了14批炒王不留行标准汤剂,并对其开展质量研究,建立了炒王不留行标准汤剂质量评价方法。以王不留行黄酮苷为指标进行含量测定,并计算其从饮片到标准汤剂的转移率;测定出膏率及pH,同时建立了HPLC特征图谱。研究结果显示,14批合格的炒王不留行标准汤剂中王不留行黄酮苷转移率为58.98%~93.94%,干膏得率8.67%~17.83%,pH 5.55~6.44,指纹图谱分析确定了9个共有峰,对14批王不留行饮片标准汤剂分别进行相似度评价,其相似度均大于0.96。该研究中炒王不留行标准汤剂制备方法规范,标准汤剂指纹图谱相似度高,其方法精密度、稳定性、重复性及加样回收率均良好,所建立的质量评价方法便捷、可靠,可为炒王不留行标准汤剂、配方颗粒及相关经典名方制剂(汤剂)质量控制提供参考。
According to the preparation principle of standard decoction of Chinese herbal medicines, fourteen batches of fried Vaccariae Semen decoction were prepared in this study and the quality research was carried out to establish a quality evaluation method for the standard decoction of fried Vaccariae Semen. The contents of vaccarin were determined, and its transfer rate from decoction piece to standard decoction was calculated. The extract rate and pH value were measured, and HPLC fingerprint method was established for analysis. The results of the 14 batches of samples revealed that the transfer rates of vaccarin were between 58.98%-93.94%; the extract rates were between 8.67%-17.83%, and the pH values were between 5.55-6.44. Moreover, 9 common chromatographic peaks were identified in fingerprints analysis. The similarities of the 14 batches of samples were analyzed and compared, and the results showed that the similarities were all higher than 0.96. In this study, the preparation process for fried Vaccariae Semen standard decoction was standard, with high similarities in fingerprint. A convenient and reliable method of comprehensive quality evaluation was established in this study, with a high precision, stability and repeatability, which can provide a reference for the quality control of fried Vaccariae Semen standard decoction, dispensing granule and related Chinese classical formulas(decoction).

关键词(KeyWords): 炒王不留行;标准汤剂;指纹图谱;王不留行黄酮苷
fried Vaccariae Semen;standard decoction;fingerprints;vaccarin

Abstract:

Keywords:

基金项目(Foundation): 国家重点研发计划项目(2018YFC1707002);; 陕西国际商贸学院中药质量标志物创新团队项目(SSY18TD02)

作者(Author): 王珊;刘博男;吕新林;杨娇艳;张永欣;梁曜华;史辑;朱晶晶;
WANG Shan;LIU Bo-nan;LYU Xin-lin;YANG Jiao-yan;ZHANG Yong-xin;LIANG Yao-hua;SHI Ji;ZHU Jing-jing;School of Pharmacy, Shaanxi Institute of Internal Trade & Commerce;Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences;School of Pharmacy, Liaoning University of Traditional Chinese Medicine;Anhui University of Chinese Medicine;

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DOI: 10.19540/j.cnki.cjcmm.20200602.302

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