中国中药杂志

2013, v.38(17) 2882-2885

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藏药材“白花刺参”抑制NO生成作用的谱效相关分析
Spectrum-effect correlation analysis of traditional Tibetan medicine ″Morina nepalensis″ on nitric oxide production inhibition

罗培;刘圆;吕露阳;张志锋;
LUO Pei;LIU Yuan;LV Lu-yang;ZHANG Zhi-feng;State Key Laboratory for Quality Research of Chinese Medicines,Macau University of Science and Technology;Ethnic Pharmaceutical Institute,Southwest University for Nationalities;

摘要(Abstract):

目的:研究白花刺参HPLC-ELSD的化学指纹与抗一氧化氮(NO)生成作用的相关性,为确定抗炎活性成分推荐质量控制指标提供依据。方法:色谱柱为Eclipse XDB C18柱(4.6 mm×150 mm,5μm);流动相为水-乙腈,梯度洗脱,体积流量为1 mL·min-1;柱温35℃;ELSD漂移管温度为104℃;N2气体流速为2.8 L·min-1;进样量10μL。测定10批白花刺参的HPLC-ELSD指纹图谱和对脂多糖诱导巨噬细胞生成NO的抑制作用,采用相似度分析和偏最小二乘法对HPLC-ELSD指纹图谱与抗NO生成作用进行相关分析。结果:在10批药材色谱指纹图谱中,相似度均在0.90以上,确定15个共有峰,其中7个色谱峰与抗炎活性呈显着正相关,可被选作质量控制指标。结论:该指纹图谱检测方法方便,重复性好,可用于白花刺参药材质量控制和抗炎活性评价的实验依据。
Objective: To establish an HPLC-ELSD fingerprint of the whole herbs of Morina nepalensis and perform the correlation analysis of chemical components of the herb and nitric oxide(NO) production inhibition.Method: HPLC-ELSD assay was performed to evaluate 10 batches of M.nepalensis herbs.The chromatographic conditions were as following: Eclipse XDB C 18 column(4.6 mm ×150 mm,5 μm),water(A) and acetonitrile(B) as a gradient mobile phases,flow rate 1.0 mL·min-1,and column temperature at 35 ℃.Evaporative light-detection conditions: atomization temperature at 104 ℃,the flow rate of N2 2.8 L·min-1and 10 μL sample injection.Chromatographic fingerprint was developed,and the inhibition activity of production of NO in lipopolysaccharide-induced macrophages was also analyzed.The similarity and correlation analysis between the HPLC-ELSD fingerprints and NO production inhibition were carried out by PLS method.Result: The common mode for M.nepalensis herb fingerprint was established,including 15 common characteristic peaks.Among them,7 peaks were positively correlated with the NO production inhibition.According to the assessment on the similarity of 10 batches of samples,a similarity of over 0.90 were shown in HPLC-ELSD fingerprint and all samples were separated into two groups.Conclusion: This method can be used to assess the quality of M.nepalensis,which provides a reliable method for scientific assessment and quality control.

关键词(KeyWords): 白花刺参;HPLC-ELSD;指纹图谱;相关分析;抗炎活性
Morina nepalensis;HPLC-ELSD;chromatographic fingerprint;similarity analysis;anti-inflammation

Abstract:

Keywords:

基金项目(Foundation): 国家科技支撑计划项目(2012BAI27B07);; 澳门特别行政区科学技术发展基金项目(073/2011/A3)

作者(Author): 罗培;刘圆;吕露阳;张志锋;
LUO Pei;LIU Yuan;LV Lu-yang;ZHANG Zhi-feng;State Key Laboratory for Quality Research of Chinese Medicines,Macau University of Science and Technology;Ethnic Pharmaceutical Institute,Southwest University for Nationalities;

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