中国中药杂志

2013, v.38(07) 1026-1029

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虎耳草乙酸乙酯部位高效液相色谱指纹图谱研究
HPLC fingerprint of ethyl acetate extraction of Saxifraga stolonifera

周美;陈华国;先春;黄志金;周欣;
ZHOU Mei1,2,CHEN Hua-guo1,2,XIAN Chun1,2,HUANG Zhi-jin1,2,ZHOU Xin1,2*(1.The Research Center for Quality Control of Natural Medicine,Guizhou Normal University,Guiyang 550001,China;2.Key laboratory for Information System of Mountainous Areas and Protection of Ecological Environment,Guizhou Normal University,Guiyang 550001,China)

摘要(Abstract):

目的:建立虎耳草药材的乙酸乙酯部位HPLC指纹图谱分析方法。方法:以Diamonsil C18色谱柱(4.6mm×250mm,5μm),流动相乙腈(A)-0.05%磷酸水溶液(B),梯度洗脱,流速1.0mL·min-1,检测波长256nm,柱温30℃。结果:建立了虎耳草药材的乙酸乙酯部位指纹图谱,确定了15个共有峰,并用对照品指认了7个峰,精密度和重复性实验中各共有峰相对保留时间RSD均小于3%,11批不同产地虎耳草相似度存在差异。结论:虎耳草药材乙酸乙酯部位指纹图谱的建立为药材质量控制提供了科学依据。
Objective:To establish an HPLC fingerprint of ethyl acetate extraction of Saxifraga stolonifera.Method:The HPLC analysis was performed on a Diamonsil C18(4.6 mm × 250 mm,5 μm) column with isocratic elution of acetonitrile-0.05% phosphoric acid at a flow rate of 1.0 mL·min-1.The detection wavelength was set at 256 nm and the column temperature was set at 30 ℃.Result:The HPLC fingerprint of ethyl acetate extraction of S.stolonifera has been established.There were fifteen common peaks,seven of which were identified by reference substances.The RSD of relative retention time was less than 3% in the precision and repeated tests.Eleven samples from different area can be distinguished from their fingerprints.Conclusion:This method is reasonable and reliable and can be used for quality control of S.stolonifera.

关键词(KeyWords): 虎耳草;高效液相色谱;指纹图谱
Saxifraga stolonifera;HPLC;fingerprint

Abstract:

Keywords:

基金项目(Foundation): 贵州省中药现代化科技产业研究开发专项(黔科合中药字[2010]5020号);; 贵州省科技创新人才团队建设项目(黔科合人才团队(2011)4008);; 贵阳市科学技术计划项目([2010]筑科合同字第1-中-22号);; 贵州省中药材现代产业技术体系建设专项(GZ-CYTX-02);; 贵州省教育厅特色重点实验室项目(黔科合KY[2012]005号)

作者(Author): 周美;陈华国;先春;黄志金;周欣;
ZHOU Mei1,2,CHEN Hua-guo1,2,XIAN Chun1,2,HUANG Zhi-jin1,2,ZHOU Xin1,2*(1.The Research Center for Quality Control of Natural Medicine,Guizhou Normal University,Guiyang 550001,China;2.Key laboratory for Information System of Mountainous Areas and Protection of Ecological Environment,Guizhou Normal University,Guiyang 550001,China)

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