中国中药杂志

2015, v.40(12) 2352-2356

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枳壳中黄酮类成分的分离与鉴定
Isolation and structural identification of flavonoids from Aurantii Fructus

丁邑强;熊英;周斌;邓敏芝;邓可众;
DING Yi-qiang;XIONG Ying;ZHOU Bin;DENG Min-zhi;DENG Ke-zhong;College of Pharmacy,Jiangxi University of Traditional Chinese Medicine;College of Pharmacy,Jiangxi Science and Technology Normal University;School of Chinese materia medica,Beijing University of Chinese Medicine;

摘要(Abstract):

常用理气药枳壳来源于芸香科柑橘属酸橙Citrus aurantium及其栽培变种的干燥未成熟果实。研究枳壳中的化学成分,采用硅胶,Sephadex LH-20,HW-40凝胶,ODS柱色谱,制备HPLC以及制备TLC等方法进行分离纯化,根据理化性质和波谱数据如NMR,MS等鉴定化合物的结构。从枳壳乙醇提取物的石油醚层和乙酸乙酯层中分离得到14个黄酮类化合物,包括4个黄酮苷和10个多甲氧基黄酮,分别鉴定为(2R)和(2S)-6″-O-乙酸基洋李苷(1,2),柚皮素-7-O-β-D-葡萄糖苷(3)、5,7,4'-三羟基-8,3'-二甲氧基黄酮-3-O-6″-(3-羟基-3-甲基戊二酸单酯)-β-D-葡萄糖苷(4)、4'-羟基-5,6,7-三甲氧基黄酮(5)、柚皮黄素(6)、川陈皮素(7)、甜橙素(8)、5,6,7,4'-四甲氧基黄酮(9)、5,7,8,4'-四甲氧基黄酮(10)、3,5,6,7,8,3',4'-七甲氧基黄酮(11)、桔皮素(12)、5-去甲川陈皮素(13)、5-羟基-6,7,3',4'-四甲氧基黄酮(14),其中化合物1为新化合物,3~5为首次从酸橙植物中分离得到。
Aurantii Fructus is the dried and immature fruit of Citrus aurantium and its cultivars. To investigate the chemical constituents of Aurantii Fructus,the separation and purification of constituents were performed by column chromatography on silica gel,LH-20,HW-40,ODS,PHPLC and PTLC. Fourteen flavonoids,including four flavone glycosides and ten polymethoxyflavones( PMFs)were isolated from the Et OAc fraction and Petroleum ether fraction of Aurantii Fructus and their structures were identified by physicochemical properties and spectral data( NMR and MS) as(2R)-and(2S)-6″-O-acetylprunin(1,2),naringenin-7-O-β-D-glucopyranside(3),5,7,4'-trihydroxy-8,3'-dimethoxyflavone-3-O-6″-(3-hydroxyl-3-methylglutaroyl)-β-D-glucopyranoside(4),4'-hydroxy-5,6,7-trimethoxyflavone(5),natsudaidain(6),nobiletin(7),sinensetin(8),5,6,7,4'-tetramethoxyflavone(9),5,7,8,4'-tetramethoxyflavone(10),3,5,6,7,8,3',4'-heptamethoxyflavone(11),tangeretin(12),5-demethyl nobiletin(13),and 5-hydroxy-6,7,3',4'-tetramethoxyflavone(14). Compound 3-5 s were isolated from this plant for the first time and compound 1 was a new one.

关键词(KeyWords): 枳壳;黄酮;结构鉴定
Aurantii Fructus;flavonoids;structure identification

Abstract:

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基金项目(Foundation): 国家自然科学基金项目(81060331);; 江西中医药大学道地药材专项(ZX1022)

作者(Author): 丁邑强;熊英;周斌;邓敏芝;邓可众;
DING Yi-qiang;XIONG Ying;ZHOU Bin;DENG Min-zhi;DENG Ke-zhong;College of Pharmacy,Jiangxi University of Traditional Chinese Medicine;College of Pharmacy,Jiangxi Science and Technology Normal University;School of Chinese materia medica,Beijing University of Chinese Medicine;

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