中国中药杂志

2014, v.39(05) 860-866

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新疆桃仁HPLC-UV指纹图谱研究及在地区分类中的应用
Quality assessment and classification of Persicae Semen based on HPLC-UV fingerprint

云琦;刘青旺;马小华;高晓黎;周建平;
YUN Qi;LIU Qing-wang;MA Xiao-hua;GAO Xiao-li;ZHOU Jian-ping;Department of Pharmaceutics,China Pharmaceutical University;College of Traditional Medicine,China Pharmaceutical University;Xinjiang Uygur Autonomous Region Food and Drug Administration;College of Pharmacy,Xinjiang Medical University;

摘要(Abstract):

该研究建立了新疆桃仁HPLC-UV指纹图谱测定方法,方法采用Shimadzu Inertsil C18(4.6 mm×250 mm,5μm)色谱柱,以水-乙腈线性梯度洗脱,流速1.0 mL·min-1,柱温35℃,检测波长210 nm。指纹图谱中的9个色谱峰利用UPLC-QTOF-MS得到了指认,对41批样品指纹图谱进行聚类分析(HCA)、偏最小二乘法判别分析(PLS-DA)和相似度分析(SA),新疆桃仁可分为2类,其中6个成分为分类标志物。所建立指纹图谱方法简便、稳定、可行,可用于新疆桃仁的质量控制及分类。
Persicae Semen( PS),a traditional Chinese medicine,has been widely used for the syndrome of blood stasis in China since the Eastern Han Dynasty. In the present study,we developed an HPLC-UV fingerprint analysis method for the quality control of PS. The HPLC fingerprint was performed on Shimadzu Inertsil C18column( 4. 6 mm × 250 mm,5 μm) at 35 ℃. The mobile phases were composed of acetonitrile and water using a gradient elution. The flow rate was 1. 0 mL·min- 1,and the detection wavelength was set at 210 nm. The fingerprint method was validated according to the Guidelines for Traditional Chinese Medicine Injection Fingerprint, and applied to determine 41 batches representative herbs collected from Xinjiang of China. The chromatographic peaks were characterized by UPLC-Q-TOF-MS,and nine of them were identified by comparison with the literature and / or reference standards. In order to classify and assess the samples,hierarchical clustering analysis and partial least square discriminant analysis were performed based on the common chromatographic peaks,and the samples were geographically classified into two classes,with six chemical compounds as classification markers which were significantly different between the two classes( P < 0. 05).

关键词(KeyWords): 桃仁;指纹图谱;高效液相色谱法;质谱;维药
Persicae Semen;fingerprint;HPLC;MS;Uygur medicine

Abstract:

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基金项目(Foundation): 新疆乌鲁木齐科技攻关计划项目(G121120002);; 创新基金项目(CX11125W)

作者(Author): 云琦;刘青旺;马小华;高晓黎;周建平;
YUN Qi;LIU Qing-wang;MA Xiao-hua;GAO Xiao-li;ZHOU Jian-ping;Department of Pharmaceutics,China Pharmaceutical University;College of Traditional Medicine,China Pharmaceutical University;Xinjiang Uygur Autonomous Region Food and Drug Administration;College of Pharmacy,Xinjiang Medical University;

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