中国中药杂志

2019, v.44(24) 5358-5362

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人参内生细菌F1对人参根系分泌物的化学趋向性响应
Chemotactic response of ginseng endophyte to ginseng root exudates

张欣欣;张爱华;雷锋杰;才丽;徐周洋;刘志庆;张连学;
ZHANG Xin-xin;ZHANG Ai-hua;LEI Feng-jie;CAI Li;XU Zhou-yang;LIU Zhi-qing;ZHANG Lian-xue;Jilin Province Ginseng Engineering Technology Research Center,College of Chinese Medicinal Materials,Jilin Agricultural University;

摘要(Abstract):

人参内生细菌F1是人参细菌性软腐病的潜在生物防治菌剂,为探讨其生防机制,该文采用毛细管法检测人参内生细菌F1对8种人参根系分泌物的化学趋向性,并探究不同趋化参数(浓度、时间、温度和pH)下,F1菌株对葡萄糖、甘氨酸、L-鼠李糖、L-谷氨酸等4种趋化作用较好的物质化学趋向性响应。结果显示,在相同实验条件(孵育温度25℃,孵育时间60min,趋化液质量浓度1 mg·L~(-1))下,人参内生细菌F1对所测的8种物质表现出了不同响应程度的趋化现象,对所测的糖类和氨基酸类物质具有正向趋化反应,而对人参总皂苷的趋化响应度较低。在一定参数范围内趋化性响应程度与趋化指数呈现正相关,但随着温度、pH、时间、浓度等因素不断增高,趋化效果反而降低,,F1对4种物质的最佳趋化参数分别如下:葡萄糖25℃,10 mg·L~(-1),45 min,pH 7;甘氨酸30℃,10 mg·L~(-1),75 min,pH 7; L-鼠李糖30℃,1 mg·L~(-1),30 min,p H 6; L-谷氨酸25℃,0. 1 mg·L~(-1),45 min,pH 8。趋化响应对低浓度的趋化物质更加敏感。
The ginseng endophytic bacteria F1 is a potential biocontrol agent for ginseng bacterial soft rot. In this paper,the chemotactic response of ginseng endophytic bacteria F1 on 8 kinds of sugar and amino acids was detected by capillary method to explore its biocontrol mechanism. The chemotactic response of F1 strain to 4 kinds of better chemotaxis substances such as glucose,glycine,L-rhamnoseand L-glutamic acid under parameters( concentration,time,temperature and pH) was studied. The results showed that under the same experimental conditions( incubation temperature 25 ℃,incubation time 60 min,chemotaxis concentration 1 mg·L~(-1)),ginseng endophytic bacteria F1 showed different degrees of response to the eight substances tested. The phenomenon of positive chemotaxis of the measured sugars and amino acids was obvious,and the chemotactic response to total ginsenosides was low. The degree of chemotaxis response is positively correlated with the chemotaxis index within a certain range of parameters,but as the temperature,p H,time,concentration and other factors continue to increase,the chemotaxis effect decreases,and F1 optimizes the chemotaxis of the four substances. The parameters are as follows: glucose: 25 ℃,10 mg·L~(-1),45 min,pH 7; glycine: 30 ℃,10 mg·L~(-1),75 min,pH7; L-rhamnose: 30 ℃,1 mg·L~(-1),30 min,pH 6; L-glutamic acid: 25 ℃,0. 1 mg·L~(-1),45 min,pH 8. The chemotactic response is more sensitive to low concentrations of chemotactic substances.

关键词(KeyWords): 人参内生细菌F1;化学趋向性;根系分泌物;糖;氨基酸
ginseng endophytic bacteria F1;chemotaxis;root exudates;sugar;amino acids

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基金项目(Foundation): 吉林省科技发展计划项目(20170204018YY);; 吉林省自然科学基金主题引导项目(20190201297JC)

作者(Author): 张欣欣;张爱华;雷锋杰;才丽;徐周洋;刘志庆;张连学;
ZHANG Xin-xin;ZHANG Ai-hua;LEI Feng-jie;CAI Li;XU Zhou-yang;LIU Zhi-qing;ZHANG Lian-xue;Jilin Province Ginseng Engineering Technology Research Center,College of Chinese Medicinal Materials,Jilin Agricultural University;

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