刘茂峰;谢美萍;李兰;鲁安琪;石建功;王素娟;
LIU Mao-feng;XIE Mei-ping;LI Lan;LU An-qi;SHI Jian-gong;WANG Su-juan;State Key Laboratory of Bioactive Substance and Function of Natural Medicines,Institute of Materia Medica,Chinese Academy of Medical Sciences & Peking Union Medical College;

• 1:中国医学科学院中国协和医学院药物研究院天然药物活性物质与功能国家重点实验室

摘要(Abstract)：

该研究通过对不同来源的桑白皮流浸膏进行薄层鉴别、含量测定及指纹图谱等研究,建立了合理可行的质量标准。薄层鉴别方法使用聚酰胺薄膜,以冰醋酸-水(1∶3)为展开剂,在365 nm下检视。该方法专属性好,合格样品均含有与对照药材和对照品相同的显色斑点。建立了HPLC测定桑皮苷A含量的方法,色谱条件为:甲醇-水(25∶75)为流动相;检测波长326nm;标准曲线为Y=46.965X,r=0.999 6;线性范围4.6~228 mg·L-1。14批样品中4批桑皮苷A的质量分数小于0.5 g·L-1,其余样品质量分数均大于2.0 g·L-1,建议将桑皮苷A的含量限量定为不少于1.5 g·L-1。使用HPLC梯度洗脱,利用中药色谱指纹图谱相似度评价系统对桑白皮流浸膏的指纹图谱进行评价,发现各批样品之间相似度较低,且桑白皮药材的化学多样性是影响相似度的决定因素,工艺因素影响较小,因此暂不拟列入质量标准。
A reasonable and practicable quality standard was developed for mori liquid extract from different sources by TLC,HPLC and fingerprint technology. In TLC method,the compounds were separated on polyamide film using glacial acetic acid-water( 1 ∶ 3) as mobile phase at a UV wavelength of 365 nm. All qualified samples had the spots of the same color as the control herb and substance.The RP-HPLC method was used to determine the content of mulberroside A with mobile phase of methanol-water( 25 ∶ 75) at a wavelength of 326 nm. The mulberroside A was in good linear with a regression equation of Y = 46. 965X( r = 0. 999 6) in the range of 4. 6-228 mg·L-1. In 14 batches of samples,the mulberroside A in 4 batches of them was less than 0. 5 g·L-1,and was more than 2. 0g·L-1in the other batches. It was suggested that the content limit of mulberroside A should be no less than 1. 5 g·L-1. The HPLC fingerprints were evaluated by the similarities. It has found that the similarities of different mori liquid extracts were very low and the chemical diversity of mori cortex was the major factor of similarity. Moreover,the process impact was minimal. Thus the fingerprint was not included in this quality standard.

关键词(KeyWords)： 桑白皮;流浸膏;桑皮苷A;质量标准;含量测定;指纹图谱
Mori Cortex;liquid extracts;mulberroside A;quality standard;content determination;fingerprint

Abstract:

Keywords:

基金项目(Foundation): 2015年版中国药典标准提高项目

作者(Author): 刘茂峰;谢美萍;李兰;鲁安琪;石建功;王素娟;
LIU Mao-feng;XIE Mei-ping;LI Lan;LU An-qi;SHI Jian-gong;WANG Su-juan;State Key Laboratory of Bioactive Substance and Function of Natural Medicines,Institute of Materia Medica,Chinese Academy of Medical Sciences & Peking Union Medical College;

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DOI:

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