中国中药杂志

2004, (02)

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蓝桉油对脂多糖引起的大鼠慢性支气管炎及黏蛋白高分泌的影响
Effect of Eucalyptus globulus oil on lipopolysaccharide-induced chronic bronchitis and mucin hypersecretion in rats

吕小琴,唐法娣,王砚,赵婷,卞如濂
LU Xiao-qin,TANG Fa-di,WANG Yan,ZHAO Ting,BIAN Ru-lian (Zhejiang Respiratory Drugs Research Laboratory of State Food and Drugs Administration of China,College of Medical Sciences,Zhejiang University,Hangzhou 310031,China)

摘要(Abstract):

目的 :观察蓝桉油对脂多糖引起的大鼠慢性支气管炎及黏蛋白高分泌的影响。方法 :采用一次性气管内注入 0 .2mg脂多糖复制大鼠慢性支气管炎模型 ,观察 3周后的病理学改变、支气管肺泡灌洗液变化、免疫组化特点以及蓝桉油对其的影响。结果 :注入脂多糖后的病理学表现符合慢支的特征。各给药组经不同浓度蓝桉油灌胃后能减少中性粒细胞及淋巴细胞的浸润 ,降低炎症程度 ;尤其是蓝桉油高剂量组 (30 0mg·kg-1) ,能明显减少灌洗液中黏蛋白含量 ,使气管和细支气管上皮内黏蛋白MUC5ac表达的阳性染色区域和程度明显减轻 ,通过图像分析系统测得的吸光度值和黏蛋白面积 %均明显低于模型组。结论 :蓝桉油对脂多糖引起的大鼠慢性支气管炎具有一定的抗炎作用 ,并能抑制其气道黏蛋白高分泌现象。
Objective: To study the effect of Eucalyptus globulus oil on bronchiolitis and mucin hypersecretion in chronic bronchitis induced by lipopolysaccharide in rats. Method: Rat model was established by intratracheal instillation of lipopolysaccharide 0.2 mg. Pathological changes,alteration in bronchoalveolar lavage fluid(BALF) and immunohistochemistry characters were examined after 3 weeks and the effect of E. globulus oil was observed. Result: Characters of pathological manifestations of chronic bronchitis were found after instillation of LPS. Inflammatory cell infiltration and bronchiolitis severity were significantly reduced ofter administration of E. globulus oil. Especially in 300 mg·kg -1 treated rats,there were significant decreases of mucin content in BALF and MUC5ac expression in trachea and bronchiole epithelium. Optical density and mucins area% detected by image analysis system were apparently lower than those in model group. Conclusion: E. globulus oil has the anti-inflammatory effect on chronic bronchitis induced by lipopolysaccharide in rats and the inhibitio effect on hypersecretion of airway mucins.

关键词(KeyWords): 蓝桉油;脂多糖;慢性支气管炎;黏蛋白
Eucalyptus gloulus oil;lipopolysaccharide;bronchiolitis;mucins

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基金项目(Foundation): 浙江省科技计划项目 (2 0 0 3 c3 3 0 0 8)

作者(Author): 吕小琴,唐法娣,王砚,赵婷,卞如濂
LU Xiao-qin,TANG Fa-di,WANG Yan,ZHAO Ting,BIAN Ru-lian (Zhejiang Respiratory Drugs Research Laboratory of State Food and Drugs Administration of China,College of Medical Sciences,Zhejiang University,Hangzhou 310031,China)

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