中国中药杂志

2021, v.46(06) 1490-1497

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两色金鸡菊总黄酮化学成分肠道吸收特性研究
Study on rat intestinal absorption characteristics of total flavonoids from Coreopsis tinctoria

罗莉;张玉姗;刘谢英;王淑珍;李作铭;姚新成;
LUO Li;ZHANG Yu-shan;LIU Xie-ying;WANG Shu-zhen;LI Zuo-ming;YAO Xin-cheng;School of Pharmacy,Shihezi University;Ministry of Education Key Laboratory of Xinjiang Plant Resources Utilization;

摘要(Abstract):

考察两色金鸡菊总黄酮中化学成分在大鼠不同肠段的吸收情况,采用大鼠外翻肠囊模型,选择矢车菊素-3-O-β-D-葡萄糖苷、绿原酸、黄诺马苷、栎草亭-7-O-β-D-吡喃葡萄糖苷、异奥卡宁、马里苷和3,5-O-二咖啡酰奎宁酸7种两色金鸡菊总黄酮主要化学成分作为考察对象,评价各成分在不同肠段的吸收特性。结果显示,两色金鸡菊总黄酮不同浓度时矢车菊素-3-O-β-D-葡萄糖苷等7种成分在各肠段的吸收均符合零级吸收速率;绿原酸、黄诺马苷、栎草亭-7-O-β-D-葡萄糖苷、异奥卡宁和3,5-O-二咖啡酰奎宁酸的吸收速率常数(K_a)均随给药浓度的增加而增加(P<0.05),提示这5种成分的肠道吸收为被动扩散;而矢车菊素-3-O-β-D-葡萄糖苷和马里苷的K_a浓度依赖性较弱,可能是一种主、被动同时存在的吸收方式。不同肠道部位的吸收结果表明,回肠可能是矢车菊素-3-O-β-D-葡萄糖苷、绿原酸、黄诺马苷、栎草亭-7-O-β-D-葡萄糖苷、马里苷和3,5-O-二咖啡酰奎宁酸的主要吸收部位,空肠可能是异奥卡宁的主要吸收部位。不同肠道部位对两色金鸡菊总黄酮中各成分的吸收具有选择性,采用外翻肠囊法可以评价两色金鸡菊总黄酮中多组分的肠吸收特性。
The rat everted intestinal sac model was adopted to investigate the absorption of total flavonoids from Coreopsis tinctoria in different intestinal segments. Cyaniding-3-O-β-D-glucoside, chlorogenic acid, flavanomarein, quercetagetin-7-O-β-D-glucoside, iso-okanin, marein and 3,5-dicaffeoylquinic acid which as the major chemical components of total flavonoids from C. tinctoria were selec-ted as the study objects to evaluate the absorption characteristics of each component in different intestinal segments. The results showed that the absorption of seven components of total flavonoids at different intestinal segments was in consistent with zero order absorption rate. The K_a of chlorogenic acid, flavanomarein, quercetagetin-7-O-β-D-glucoside, isookanin and 3,5-dicaffeoylquinic acid increased with increasing of concentration of total flavonoids(P<0.05), indicating that the intestinal absorption of these five components was passive transport. The K_a of cyaniding-3-O-β-D-glucoside and marein showed a weak concentration dependence, suggesting that the absorption of them may be an positive and passive co-existing mode. The result of absorption in different intestinal segments showed that cyaniding-3-O-β-D-glucoside, chlorogenic acid, flavanomarein, quercetagetin-7-O-β-D-glucoside, marein and 3,5-dicaffeoylquinic acid were mainly absorbed in ileum, while isookanin was mainly absorbed in jejunum. The total flavonoids of C. tinctoria are selectively absorbed in intestinal tract, the rat everted intestinal sac model can be used to evaluate the multi-component intestinal absorption characteristics of total flavonoids from C. tinctoria.

关键词(KeyWords): 两色金鸡菊;总黄酮;多组分;外翻肠囊;肠吸收
Coreopsis tinctoria;total flavonoids;multiple component;everted intestinal sac;intestinal absorption

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(81660641)

作者(Author): 罗莉;张玉姗;刘谢英;王淑珍;李作铭;姚新成;
LUO Li;ZHANG Yu-shan;LIU Xie-ying;WANG Shu-zhen;LI Zuo-ming;YAO Xin-cheng;School of Pharmacy,Shihezi University;Ministry of Education Key Laboratory of Xinjiang Plant Resources Utilization;

Email:

DOI: 10.19540/j.cnki.cjcmm.20200706.202

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