中国中药杂志

2010, v.35(23) 3188-3191

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RP-HPLC同时测定民族药红药中苯乙醇苷类化合物plantainoside D和毛蕊花糖苷
Simultaneous determination of plantainoside D and verbascoside from stem of Chirita longgangensis var. hongyao by RP-HPLC

王满元;樊媛洁;张静;龚慕辛;
WANG Manyuan1,FAN Yuanjie2,ZHANG Jing3,GONG Muxin1(1.School of Traditional Chinese Medicine,Capital Medical University,Beijing 100069,China;2.School of Biochemical and Pharmaceutical Sciences,Capital Medical University,Beijing 100069,China;3.Yang Fangdian Beijing Hospital,Beijing 100038,China)

摘要(Abstract):

目的:建立同时测定民族药红药中苯乙醇苷类化合物plantainoside D和毛蕊花糖苷含量的方法。方法:采用反相高效液相色谱法,用Agilent C18(4.6 mm×250 mm,5μm)柱,以乙腈-1%醋酸溶液(16∶84)为流动相,流速1.0 mL.min-1,检测波长332 nm,柱温30℃。结果:RP-HPLC测定的plantainoside D和毛蕊花糖苷线性范围分别在6.250~100.0 mg.L-1(r=0.999 8)和12.50~500.0 mg.L-1(r=0.999 8),平均回收率分别为101.3%,100.8%,RSD分别为2.6%,2.2%(n=9)。结论:方法简便,精密度、重复性良好,结果准确可靠,可以作为红药药材及相关制剂质量控制的一个有效方法。
Objective: To establish a RP-HPLC method for simultaneous determination of phenylethanoid glycosides plantainoside D and verbascoside in Chirita longgangensis var.hongyao.Method: The analysis was performed on a Agilent C18 column(4.6 mm×250 mm,5 μm) with CH3CN-1%HAc(16∶84)as mobile phase at a flow rate of 1.0 mL·min-1,and at a column temperature of 30 ℃.The detection wave length was 332 nm.Result: The linear ranges of calibration of plantainoside D and verbascoside were 3.125-100.00 mg·L-1(r=0.999 8) and 25.00-500.0 mg·L-1(r=0.999 8).The average recoveries were 101.3% and 100.8% with RSD of 2.6%and 2.2%(n=9),respectively.Conclusion: The method is simple,accurate,reliable and can be used for the quality evaluation of C.longgangensis var.hongyao and its preparation.

关键词(KeyWords): RP-HPLC;红药;苯乙醇苷类化合物;plantainoside D;毛蕊花糖苷
RP-HPLC;Chirita longgangensis var.hongyao;phenylethanoid glycoside;plantainoside D;verbascoside

Abstract:

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基金项目(Foundation): 北京市优秀人才培养资助项目(20061D0501800256)

作者(Author): 王满元;樊媛洁;张静;龚慕辛;
WANG Manyuan1,FAN Yuanjie2,ZHANG Jing3,GONG Muxin1(1.School of Traditional Chinese Medicine,Capital Medical University,Beijing 100069,China;2.School of Biochemical and Pharmaceutical Sciences,Capital Medical University,Beijing 100069,China;3.Yang Fangdian Beijing Hospital,Beijing 100038,China)

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