中国中药杂志

2020, v.45(19) 4574-4581

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人参皂苷糖基转移酶基因研究概述
Overview of research in ginsenosides glycosyltransferase

林廷文;杜金法;黄丽瑾;陆续;
LIN Ting-wen;DU Jin-fa;HUANG Li-jin;LU Xu;State Key Laboratory of Natural Medicines, School of Traditional Chinese Pharmacy,China Pharmaceutical University;

摘要(Abstract):

糖基转移酶(UGTs)是人参皂苷生物合成中的关键酶,可催化糖基从供体分子转移到受体分子,形成各种具有生物活性的糖苷类化合物。随着高通量测序技术的发展,大量的药用植物基因组和转录组序列被解析,越来越多的UGTs基因得以发掘与验证。UGTs基因常见的发掘手段有差异表达分析、同源序列筛选、基因簇筛选、化学蛋白质组筛选等。该文总结了人参属3种名贵中药材三七、人参、西洋参中UGTs基因筛选和功能验证的研究进展,为天然产物中UGTs基因的研究提供参考。
Glycosyltransferases(UGTs) are key enzymes in the biosynthesis of ginsenosides, which can catalyze the transfer of glycans from donor molecules to acceptor molecules, and form a variety of biologically active glycoside compounds. With the development of high-throughput sequencing technology, a large number of medicinal plant genome and transcriptome sequences have been catalyzed, while more and more UGT genes have been discovered and verified. The methods of discovering UGT genes include differential expression analysis, homologous sequence screening, gene cluster screening, chemical proteome screening. This paper summarized the research progress of UGT genes screening and functional verification of three valuable Panax medicinal materials P. notoginseng, P. ginseng, and P. quinquefolius, so as to provide a reference for the study of UGT genes in natural products.

关键词(KeyWords): 人参皂苷;糖基转移酶;生物合成;基因筛选
ginsenosides;glycosyltransferase;biosynthesis;gene screening

Abstract:

Keywords:

基金项目(Foundation): 国家重点研发计划项目(2019YFC1711000);; 国家自然科学基金项目(81973414);; 江苏省研究生研究与实践创新计划项目(KYCX19_0650)

作者(Author): 林廷文;杜金法;黄丽瑾;陆续;
LIN Ting-wen;DU Jin-fa;HUANG Li-jin;LU Xu;State Key Laboratory of Natural Medicines, School of Traditional Chinese Pharmacy,China Pharmaceutical University;

Email:

DOI: 10.19540/j.cnki.cjcmm.20200508.602

参考文献(References):

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