中国中药杂志

2004, (02)

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盾叶薯蓣成熟叶片植株再生的研究
Studies on plantlet regeneration from the mature leaves of Dioscorea zingiberensis

陈永勤,樊晋宇,易飞,傅运生,罗忠训
CHEN Yong-qin,FAN Jin-yu,YI Fei,FU Yun-sheng,LUO Zhong-xun (Faculty of Life Sciences,Hubei University,Wuhan 430062,China)

摘要(Abstract):

目的 :以成熟叶片为外植体 ,建立一套盾叶薯蓣Dioscoreazingiberensis的快速繁殖技术。方法 :以大量元素减半的MS培养基为基本培养基 ,通过研究BA ,NAA和IBA对愈伤组织诱导、不定芽分化、不定芽增殖和生根的影响找出相应的最佳激素组合。结果与结论 :在含 1.0~ 5 .0mg·L-1BA和 1.0~ 2 .0mg·L-1NAA的培养基上 ,80 %以上的叶片可以在 6 0d内形成愈伤组织 ;愈伤组织转到含 2 .0~ 5 .0mg·L-1BA和 0 .2~ 0 .5mg·L-1NAA的培养基上后 ,6 0 %以上的愈伤组织可以在 5 0d内分化出不定芽 ;在含 2 .0mg·L-1IBA的培养基上 ,10 0 %的不定芽可在2 0d内长出 4~ 6条不定根。再生植株移栽后 ,生长健壮 ,成活率达 80 %以上。
Objective: To establish a protocol of rapid clonal propagation of Dioscorea zingiberensis using mature leaves as explants. Method: Out the optimal hormone combinations for callus induction,adventitious bud initiation,adventitious bud multiplication and rooting were found out by using MS medium with the macroelements at half strength as basal medium and studying the effects of BA,NAA and IBA on the processes. Result and Conclusion: More than 80% of leaf explants formed callus when they were cultured on the media containing 1.0~5.0 mg·L -1 BA and 1.0~2.0 mg·L -1 NAA for 60 days;more than 60% of calli initiated adventitious buds within 50 days after they were transferred to the media containing 2.0~5.0 mg·L -1 BA and 0.2~0.5 mg·L -1 NAA;all the adventitious buds rooted well after they were planted on the medium with 2.0 mg·L -1 IBA for 20 days;the regenerated plantlets grew vigorously after they were transplanted and the survival rate was up to 80%.

关键词(KeyWords): 盾叶薯蓣;叶片培养;愈伤组织;不定芽;离体无性繁殖
Dioscorea zingiberensis; leaf culture; callus; adventitious bud; in vitro clonal propagation

Abstract:

Keywords:

基金项目(Foundation): 湖北省生物化学与分子生物学重点学科基金

作者(Author): 陈永勤,樊晋宇,易飞,傅运生,罗忠训
CHEN Yong-qin,FAN Jin-yu,YI Fei,FU Yun-sheng,LUO Zhong-xun (Faculty of Life Sciences,Hubei University,Wuhan 430062,China)

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