中国中药杂志

2012, v.37(20) 3092-3096

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不同栽培品种毛橘红药材HPLC指纹图谱
HPLC fingerprint of Citri Grandis Exocarpium from different cultivars

王莲婧;林励;魏航;佘丹;黄小龙;
WANG Lian-jing,LIN Li,WEI Hang,SHE Dan,HUANG Xiao-long (Guangzhou University of Traditional Chinese Medicine,Guangzhou 510006,China)

摘要(Abstract):

目的:探讨化州柚栽培品种间的质量差异,为寻找良种提供科学依据。方法:采用HPLC建立化州市绿色生命有限公司GAP基地不同栽培品种毛橘红药材指纹图谱,采用国家药典委员会"中药色谱指纹图谱相似度评价系统2004 A版"软件进行相似度分析及品种判定。结果:不同栽培品种样品指纹图谱整体特征相似,18个共有峰峰面积相似度在0.938~0.998,利用相似度软件判定栽培品种成功率为92%。结论:应用该法可较好地判别毛橘红药材质量优劣及品种来源,并可为化州柚良种寻找提供技术手段和科学依据。
Objective: To study the quality difference among Citrus grandis ′ Tomentosas′ of different cultivars,in order to provide scientific basis for seeking for fine breeds.Method: The HPLC fingerprints were established for C.grandis ′ Tomentosa′ of different cultivars in the GAP base of Huazhou Green Life Co.,Ltd.The software of similarity evaluation system of traditional Chinese medicine HPLC fingerprints 2004A edition of Chinese Pharmacopoeia Commission was adopted for the similarity analysis and judgment of cultivars.Result: The fingerprints showed similar general characteristics of samples of different cultivars.Specifically,the similarity of areas of the 18 common peaks ranged between 0.938-0.998.The success rate of judging cultivars using similarity software stood at 92%.Conclusion: This method can be applied to better identify quality and source of cultivars of C.grandis ′ Tomentosa′,and provide technical measures and scientific basis for seeking for fine breeds of Citri Grandis Exocarpium.

关键词(KeyWords): 毛橘红;指纹图谱;不同栽培品种;质量控制;种质资源
Citri Grandis Exocarpium;HPLC fingerprint;different cultivars;quality control;germplasm resource

Abstract:

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基金项目(Foundation): 国家“十二五”科技支撑计划项目(2011BAI01B02)

作者(Author): 王莲婧;林励;魏航;佘丹;黄小龙;
WANG Lian-jing,LIN Li,WEI Hang,SHE Dan,HUANG Xiao-long (Guangzhou University of Traditional Chinese Medicine,Guangzhou 510006,China)

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