中国中药杂志

2019, v.44(23) 5174-5183

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灰树花多糖调节动物疾病模型体内免疫功能作用的Meta分析
Meta-analysis on effect of Grifola frondosa polysaccharide in regulating in vivo immunoregulatory function on animal disease models

张婷;赵霏;武凯楠;贾瑜;廖绪亮;杨丰文;张俊华;马彬;
ZHANG Ting;ZHAO Fei;WU Kai-nan;JIA Yu;LIAO Xu-liang;YANG Feng-wen;ZHANG Jun-hua;MA Bin;Evidence Based Medicine Center,School of Basic Medical Sciences,Lanzhou University;Key Laboratory of Evidence-based Medicine and Clinical Transformation in Gansu Province;Medical College,Northwest University for Nationalities;the First Clinical Medical College,Lanzhou University;School of Stomatology,Lanzhou University;Evidence-Based Medicine Center,Tianjin University of Traditional Chinese Medicine;

摘要(Abstract):

该研究采用计算机检索PubMed,Embase,Web of Scinece,CNKI,CBM和万方数据库,检索时间截止2018年2月。2位研究者独立筛选文献,并采用SYRCLE动物实验偏倚风险评估工具进行质量评价。使用R软件对灰树花多糖调节动物疾病模型体内免疫调节作用进行Meta分析。最终纳入20篇动物实验研究。Meta分析结果显示,细胞免疫中,灰树花多糖可增强小鼠效应T细胞、自然杀伤细胞、巨噬细胞的增殖活性。灰树花多糖组CD4+T细胞、CD8+T细胞、NK细胞、巨噬细胞的百分比{(MD=1. 89,95%CI [0. 94,2. 83],P<0. 000 1)、(MD=8. 46,95%CI [5. 93,11. 00],P<0. 000 1)、(MD=2. 67,95%CI[0. 23,5. 11],P=0. 03)、(MD=14. 09,95%CI [0. 84,27. 34],P=0. 04)}均高于对照组。体液免疫中,灰树花多糖可增加TNF-α和INF-γ分泌量。灰树花多糖组TNF-α(SMD=15. 92,95%CI [9. 07,22. 76],P<0. 000 1)和INF-γ(SMD=5. 34,95%CI[3. 42,7. 26],P<0. 000 1)的分泌量均高于对照组。灰树花多糖可从增强小鼠免疫细胞(CD4+T细胞、CD8+T细胞、NK细胞、巨噬细胞)增殖活性及增加细胞因子(TNF-α,INF-γ)分泌量2个方面调节免疫功能,但今后的研究有必要在免疫细胞特异性表面标志的选择、灰树花多糖给药方式等多个方面进一步规范,以降低研究间较大的异质性。同时,更需重视实验设计、实施和充分报告,特别是能够促进动物实验注册制定的建立和实施,以提升动物实验研究全过程的透明度及质量,最终提高基础研究的利用价值,为基础研究向临床研究转化提供可靠理论基础。
The study aimed to explore the in vivo immunoregulatory function of Grifola frondosa polysaccharide( GFP) on animal disease models. Databases of PubMed,Embase,Web of Scinece,CNKI,CBM and Wan Fang Data were searched from the date of their establishment to February 2018. Two reviewers independently screened included studies and evaluated their quality by using SYRCLE's risk of bias tool. R software was used to analyze the data. Finally,20 animal experiment studies were included. According to Metaanalysis. For cellular immunity,GFP could effectively enhance the proliferation of effect or T cells,natural killer cells and macrophages in mice. The percentage of CD4+T cells( MD = 1. 89,95% CI [0. 94,2. 83],P < 0. 000 1),CD8+T cells( MD = 8. 46,95% CI[5. 93,11. 00],P<0. 000 1),NK cells( MD= 2. 67,95% CI [0. 23,5. 11],P= 0. 03),and macrophages( MD= 14. 09,95% CI[0. 84,27. 34],P= 0. 04) were all higher than those in control group. For humoral immunity,GFP could increase the secretion of TNF-α and INF-γ. The secretion of TNF-α( SMD = 15. 92,95% CI [9. 07,22. 76],P<0. 000 1) and INF-γ( SMD = 5. 34,95% CI[3. 42,7. 26],P<0. 000 1) were all higher than those in control group. In conclusion,GFP could regulate immunologic function by enhancing the proliferation activity of immune cells( CD4+T cells,CD8+T cells,NK cells and macrophages) and the secretion of immune factors( TNF-α and INF-γ) . However,it is necessary to further standardize the selection of specific surface markers of immune cells and the administration of GFP,in order to reduce the heterogeneity among the studies. At the same time,more attention shall be paid to experimental design,implementation and full report,especially to the establishment and implementation of animal experimental registration system,so as to improve the transparency and quality of the whole process of animal experimental research,enhance the value of basic research ultimately,and provide a reliable theoretical basis for the transformation of basic research into clinical research.

关键词(KeyWords): 灰树花多糖;动物疾病模型;免疫调节功能;Meta分析
Grifola frondosa polysaccharide;animal disease models;immunoregulatory function;Meta-analysis

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(81873184,81860716);; 兰州大学中央高校基本科研项目(lzujbky-2018-98);; 兰州市城关区科技发展计划项目(2017SHFZ0019)

作者(Author): 张婷;赵霏;武凯楠;贾瑜;廖绪亮;杨丰文;张俊华;马彬;
ZHANG Ting;ZHAO Fei;WU Kai-nan;JIA Yu;LIAO Xu-liang;YANG Feng-wen;ZHANG Jun-hua;MA Bin;Evidence Based Medicine Center,School of Basic Medical Sciences,Lanzhou University;Key Laboratory of Evidence-based Medicine and Clinical Transformation in Gansu Province;Medical College,Northwest University for Nationalities;the First Clinical Medical College,Lanzhou University;School of Stomatology,Lanzhou University;Evidence-Based Medicine Center,Tianjin University of Traditional Chinese Medicine;

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