中国中药杂志

2007, (08) 706-709

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雷公藤超临界CO_2提取物的HPLC指纹图谱建立
Study on chromatographic fingerprint of extractive from Tripterygium wilfordii with supercritical carbon dioxide

周雯;李红茹;李淑芬;高石喜久;段宏泉;
ZHOU Wen1,LI Hong-ru2,LI Shu-fen2,TAKAISHI Yoshihisa3,DUAN Hong-quan1(1.School of Pharmaceutical Sciences of Tianjin,Basic Medical Research Center,Tianjin Medical University,Tianjin 300070,China;2.School of Chemical Engineering and Technology,Tianjin University,Tianjin 300072,China;3.Faculty of Pharmaceutical Sciences,University of Tokushima,Shomachi 1-78,Tokushima 770-8505,Japan)

摘要(Abstract):

目的:建立雷公藤超临界CO2萃取物的色谱指纹图谱。方法:采用高效液相色谱法对其进行测定,色谱柱:Krom asil C18(4.6 mm×250 mm,5μm),流动相乙腈-1‰磷酸水溶液进行梯度洗脱,柱温35℃,检测波长267nm,分析时间80 m in;流速1.0 mL.m in-1,测定10批超临界萃取物的色谱图,确立标准指纹图谱。结果:由此指纹图谱标示出雷公藤超临界CO2萃取物的27个共有峰,以化学对照品结合HPLC-MS鉴定了其中21峰。结论:该分析方法的稳定性、精密度、重现性较好,为雷公藤有效部位的质量控制提供了依据。
Objictive: To establish the chromatographic fingerprint of supercritical carbon dioxide extract of Tripterygium wilfordii.Method: HPLC method was applied for quality assessment of T.Wilfordii,HPLC analysis was performed on Kromasil C18(4.6 mm×250 mm,5 μm)with the mixture of acetonitrile-1‰H3PO4,as mobile phase in gradient mode.The samples were detected at UV of 267 nm with column temperature of 35 ℃,analytic time was 80 min;Flow-rate was 1.0 mL·min-1.The chromatographic fingerprint of ten batches of samples was determined,for establishing the chromatographic fingerprint of T.Wilfordii.Result: Indicating 27 peaks in common,identified 21 peaks with chemical reference and HPLC-MS,and the HPLC fingerprint was established.Conclusion: The method is steady and accurate with a good repeatability and can be used as a quality control method for T.Wilfordii.

关键词(KeyWords): 雷公藤;超临界萃取;指纹图谱
Tripterygium wilfordii;supercritical fluid extraction;fingerprint chromatography

Abstract:

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基金项目(Foundation): 天津市科委攻关培育项目(043104311);; 教育部优秀青年教师资助计划

作者(Author): 周雯;李红茹;李淑芬;高石喜久;段宏泉;
ZHOU Wen1,LI Hong-ru2,LI Shu-fen2,TAKAISHI Yoshihisa3,DUAN Hong-quan1(1.School of Pharmaceutical Sciences of Tianjin,Basic Medical Research Center,Tianjin Medical University,Tianjin 300070,China;2.School of Chemical Engineering and Technology,Tianjin University,Tianjin 300072,China;3.Faculty of Pharmaceutical Sciences,University of Tokushima,Shomachi 1-78,Tokushima 770-8505,Japan)

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