中国中药杂志

2004, (01)

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试管地黄形成过程中形态发生的研究
Morphogenesis in Formative Process of Tuberous Root in vitro from Rehmannia glutinosa

薛建平,张爱民,柳俊,徐雪峰
XUE Jian-ping 1,2 , ZHANG Ai-min 1, LIU Jun 3, XU Xue-feng 1 (1.Department of Biology, Huaibei Coal Industry Teacher's College, Huaibei 235 000 Anhui, China; 2. College of Horticulture and Forestry, Huazhong Agricultural University, Wuhan 430070, Hubei, China; 3. College of Life Sciences and Technology, Huazhong Agricultural University, Wu han 430070, China)

摘要(Abstract):

目的 :研究试管地黄形成的形态发生过程 ,比较试管地黄与自然条件下生长的地黄在形态解剖上的异同。方法 :取离体条件下和自然条件下不同阶段形成的地黄块根 ,染色后制成石蜡切片 ,于显微镜下观察。结果与结论 :试管地黄在形态解剖方面与自然条件下生长的地黄具有一致性 ,说明试管地黄在诱导过程中未发生结构上的变化。
Objective: To study the morphogenesis in f or mative process of tuberous root in vitro from Rehmannia glutinosa and co mpare the anatomical shape of tuberous root with nature term R. glutino sa. Method: Tuberous roots of different vegetal phase we re cut and dyed ,then madeinta paraffin cuts and observed microscope. Result and Conclusion: In anatomical shape,nature R. glutinosa and tuberous root were the same, which showed that no structural variation oc curred in thberous root in induced process.

关键词(KeyWords): 地黄;离体块根;形态发生;组织培养
Rehmannia glutinosa; tuberous root in vitro; morphogenesis; tissue culture

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作者(Author): 薛建平,张爱民,柳俊,徐雪峰
XUE Jian-ping 1,2 , ZHANG Ai-min 1, LIU Jun 3, XU Xue-feng 1 (1.Department of Biology, Huaibei Coal Industry Teacher's College, Huaibei 235 000 Anhui, China; 2. College of Horticulture and Forestry, Huazhong Agricultural University, Wuhan 430070, Hubei, China; 3. College of Life Sciences and Technology, Huazhong Agricultural University, Wu han 430070, China)

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