中国中药杂志

2018, v.43(09) 1789-1797

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基于网络药理学的藏药大花红景天抗氧化应激分子机制研究
Molecular mechanism of Tibetan medicine Rhodiola crenulata prevents oxidative stress through network pharmacology

范芳芳;郭非非;张雯;张艺;杨洪军;张毅;李德凤;张静;张晶晶;
FAN Fang-fang;GUO Fei-fei;ZHANG Wen;ZHANG Yi;YANG Hong-jun;ZHANG Yi;LI De-feng;ZHANG Jing;ZHANG Jing-jing;College of Ethnic Medicine,Chengdu University of Traditional Chinese Medicine;Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences;

摘要(Abstract):

该研究利用网络药理学的方法对大花红景天抗氧化应激的物质基础及分子作用机制进行研究。首先考察大花红景天肠吸收液对过氧化氢(H2O2)所致H9c2心肌细胞存活率、形态、凋亡的影响,发现大花红景天肠吸收液可增加细胞存活率、维持细胞形态和减少细胞凋亡。在此基础上,运用网络药理学的方法,从红景天中筛选出与氧化应激相关化合物133个,其中化合物靶点数目排名前20%的成分有26个,富集在43个与氧化应激功能和通路相关的核心靶点,并构建出"成分-靶点-通路-功能"网络图。结果发现,大花红景天通过红景天苷、没食子酸乙酯、儿茶素等主要成分干预细胞凋亡相关过程,如细胞死亡、一氧化氮代谢过程、氧化应激应答、线粒体机制、氧化还原过程及氧化还原相关酶的活性等生物学过程发挥H9c2心肌细胞免受氧化应激损伤的保护作用。该研究揭示了大花红景天抗氧化应激的主要药效物质及其潜在的分子机制,反映出中药大花红景天多成分、多靶点、整体调节的作用特点,为大花红景天药效物质基础及作用机制后续系统实验研究提供了有利的参考。
In this study,a computer-based network pharmacology approach was applied to investigate the potential mechanism and important components of Rhodiola crenulata in the protection of H9 c2 cells against hydrogen peroxide( H2 O2)-induced oxidative stress. The intestinal absorption liquid of R. crenulata enhanced the cell viability,maintained cell morphology and inhibited cell apoptosis in the H2 O2-induced oxidative stress in H9 c2. Then,computer-based network pharmacology was used to analyze the relevant mechanism. A total of 133 oxidative stress-related compounds were screened out; and 26 of them occupied the top 20%,and all of the compounds enriched in 43 oxidative stress-related key targets. Finally,a " compound-target-pathway-function" network was constructed. Based on the analysis of the network pharmacology,R. crenulata protected H9 c2 cells against H2 O2-induced oxidative stress probably by affecting apoptosis-related processes,such as cell death,nitric oxide metabolism,oxidative stress,mitochondrial mechanism,redox process,redox-related enzyme activty and other oxidative stress-related process. And salidroside,ethyl gallate and catechins,which were the main components of R. crenulata,played an important role in this process. Therefore,the potential mechanism and important components of R. crenulata revealed the protective effect on oxidative stress. This study shows a multicomponent,multi-target and overall regulation effect of R. crenulata on the oxidative stress,and provides a reliable reference for subsequent systematic experimental studies for the pharmacodynamic material foundation and mechanism of action R. crenulata.

关键词(KeyWords): 大花红景天;肠吸收液;网络药理学;氧化应激;药效物质;作用机制
Rhodiola crenulata;intestinal absorption solution;network pharmacology;oxidative stress;pharmacodynamic substance;mechanism

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(81203000,81603422);; 国家重点研发计划项目(2011BAI07B08);; 中医药行业科研专项(201507002);; 四川省属高校科研创新团队建设计划项目(11TD004)

作者(Author): 范芳芳;郭非非;张雯;张艺;杨洪军;张毅;李德凤;张静;张晶晶;
FAN Fang-fang;GUO Fei-fei;ZHANG Wen;ZHANG Yi;YANG Hong-jun;ZHANG Yi;LI De-feng;ZHANG Jing;ZHANG Jing-jing;College of Ethnic Medicine,Chengdu University of Traditional Chinese Medicine;Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences;

Email:

DOI: 10.19540/j.cnki.cjcmm.20180125.011

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