中国中药杂志

2019, v.44(24) 5398-5404

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炮制对天南星科4种有毒中药毒性成分凝集素蛋白的影响
Effect of processing on toxic components lectin from four kinds of Araceae toxic medicines

郁红礼;王卫;吴皓;沈敏;张元斌;李书晖;
YU Hong-li;WANG Wei;WU Hao;SHEN Min;ZHANG Yuan-bin;LI Shu-hui;Pharmacology College,Nanjing University of Chinese Medicine;Jiangsu Key Laboratory of Chinese Medicine Processing;Engineering Center of State Ministry of Education for Standardization of Chinese Medicine Processing;Suzhou Vocational Health College;

摘要(Abstract):

为研究炮制对天南星4种有毒中药半夏、掌叶半夏、天南星、白附子中毒性成分凝集素蛋白的影响,该研究采用Western blot(蛋白质印迹)法半定量分析4种有毒中药生品及其不同炮制品中凝集素蛋白含量,并以生姜汁浸泡、白矾水浸泡以及单独加热煮制的方法分别处理4种生品及4种凝集素蛋白,考察不同辅料及加热方法对凝集素蛋白的影响。结果显示,掌叶半夏、半夏、天南星和白附子生品中各凝集素蛋白的质量分数分别为7. 3%,4. 9%,2. 7%,2. 3%,清半夏中半夏凝集素的质量分数为0. 027%,姜半夏、制天南星和制白附子中均检测不到活性凝集素蛋白,表明炮制后活性凝集素含量显著降低;且随着白矾水浸泡时间或加热时间的延长,生品中凝集素含量逐渐降低。而单独生姜汁浸泡对生品中凝集素蛋白的含量几乎无影响。不同辅料及加热煮制的方法直接处理凝集素蛋白对其产生的影响与对生品产生的影响一致。故复制法以白矾浸泡及加热煮制的炮制方法可促使有毒中药中毒性成分活性凝集素蛋白的含量降低,这是有毒中药毒性下降的关键。该研究以Western blot法检测天南星科有毒中药毒蛋白凝集素蛋白的含量水平可作为炮制程度的评价方法。
The study aimed to investigate the effect of processing on lectin protein in four toxic Chinese medicines tubers of Pinellia ternata,P. pedatisecta,Arisema heterophyllum and Typhonium giganteum. Western blot was used to semi-quantitatively analyze the content of lectin in the four kinds of toxic Chinese medicines and their different processed products. Raw products and lectin were treated by heating or soaking in ginger juice or alum solution. The effects of different excipients and the heating methods on lectin proteins were investigated. The results showed that the content of lectin in raw products of P. pedatisecta,P. ternata,A. heterophyllum,and T. giganteum were 7. 3%,4. 9%,2. 7%,2. 3%,respectively. And the content of lectin in Pinelliae Rhizoma praeparatum cum alumine was 0. 027%. Lectin was not detected in the Pinelliae Rhizoma Praeparatum cum Zingibere et Alumine,Arisaematis Rhizma Praeparatum and Typhonii Rhizoma Praeparatum,which indicated that processing could significantly reduce the content of active lectin in raw products. The results also showed that with the prolongation of soaking and heating time,the content of lectin in raw products decreased gradually,while the content was almost unchanged when soaked in ginger juice alone. The effects of different excipients and heating on lectin were the same as those on raw products. Therefore,the method with alum soaking and heating can reduce the content of active lectin,which is the key to reduce the toxicity of toxic Chinese medicines. In this paper,Western blot was used to study the content of toxic protein in Araceae toxic Chinese medicines as an evaluation method of the processing degree.

关键词(KeyWords): 天南星科;毒性;炮制;凝集素蛋白
Araceae;toxic component;lectin;processing

Abstract:

Keywords:

基金项目(Foundation): 国家重点研发计划项目(2018YFC1707003);; 国家自然科学基金项目(81173549,81573605)

作者(Author): 郁红礼;王卫;吴皓;沈敏;张元斌;李书晖;
YU Hong-li;WANG Wei;WU Hao;SHEN Min;ZHANG Yuan-bin;LI Shu-hui;Pharmacology College,Nanjing University of Chinese Medicine;Jiangsu Key Laboratory of Chinese Medicine Processing;Engineering Center of State Ministry of Education for Standardization of Chinese Medicine Processing;Suzhou Vocational Health College;

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