中国中药杂志

2006, (23) 1925-1928

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“一测多评”法中药质量评价模式方法学研究
Multi-components quantitation by one marker new method for quality evaluation of Chinese herbal medicine

王智民;高慧敏;付雪涛;王维皓;
WANG Zhi-min,GAO Hui-min,FU Xue-tao,WANG Wei-hao (Institute of Chinese Materia Medica,China Academy of Chinese Medicial Sciences,Beijing 100700,China)

摘要(Abstract):

目的:建立适合于中药特点的利用1个对照品同步测定多成分的分析方法,并建立其方法学的考察模式。方法:以木通为研究对象,以药材中典型成分皂苷PJ1(sapon in PJ1)为指标,建立该成分与其他成分白木通皂苷B和白木通皂苷C(mutongsapon in B和C)间的相对校正因子,测定sapon in PJ1的含量,用校正因子计算mutong-sapon in B和C的含量;同时采用外标法实测药材中3种成分的绝对含量;采用夹角余弦法对一测多评法的计算值与外标法实测值进行比较,评价一测多评法的准确性和科学性。结果:建立了一测多评法方法学的考察模式;方法准确性评价结果表明一测多评法的计算值与外标法实测值之间没有显著性差异,实验所得的校正因子可信。结论:一测多评的研究思路在木通药材中得到验证,有望成为适合中药特点的多指标质量评价新模式。
Objective: To establish a new quality evaluation method for Chinese herbal medicine,using one chemical reference substance to calculate multi-components simultaneously.Method: Relative correction factors(RCF) of mutongsaponin B and C to saponin P_(J1),a characteristic component as marker,were calcalated in The chromatographic conditions for determination of the three saponins in Caulis Akebiae akebiae as a research model.The contents of saponin P_(J1) were determined by external standard method,and those of mutongsaponin B and C were calculated by saponin P_(J1) and their RCF.The accuracy of the new method was evaluated by comparing the calculated contents with the determined contents.Result: The analysis procedures were validated.There has been no significant difference between the calculated contents and the determined contents,according to the angle cosine value,and the calcalated RCF have confidence value.Conclusion: The method of multi-components quantitation by one marker has been verified in caulis akebiae and it is to be a new quality evaluation pattern for Chinese herbal medicines.

关键词(KeyWords): 一测多评;HPLC;木通;校正因子
multi-components quantitive by one marker; HPLC;Caulis Akebiae;relative correction factor

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作者(Author): 王智民;高慧敏;付雪涛;王维皓;
WANG Zhi-min,GAO Hui-min,FU Xue-tao,WANG Wei-hao (Institute of Chinese Materia Medica,China Academy of Chinese Medicial Sciences,Beijing 100700,China)

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