中国中药杂志

2009, v.34(02) 156-160

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指纹图谱技术考察桔梗在天王补心丸中的配伍意义
Role of Radix Platycodi in Tianwang Buxinwan decoction analyzed by fingerprints

刘萍;邹鹏;李外;孙艳;张洁;余河水;赵阳;马百平;
LIU Ping~1 ZOU Peng~2 LI Wai~1 SUN Yan~1 ZHANG jie~2 YU Heshui~2 ZHAO Yang~2 MA Baiping~(2*) (1. Pharmacy of Traditional Chinese Medicine, PLA General Hospital, Beijing 100853, China 2. Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China)

摘要(Abstract):

目的:建立天王补心丸活性部位的高效液相(HPLC)指纹图谱,从化学成分角度阐述桔梗舟楫作用的物质基础和科学内涵。方法:采用Hanbon Lichrospher C_(18)色谱柱(4.6mm×250 mm,5μm),流动相甲醇和0.012 5 mol·L~(-1)乙酸铵-醋酸水溶液线性梯度洗脱,流速为1.0 mL·min~(-1),检测器为二极管阵列检测器(PDA)与蒸发光散射检测器(ELSD)串联使用,建立指纹图谱;将全方组与缺桔梗组、桔梗组与单独桔梗组及单独酸枣仁组进行指纹图谱比较;同时比较各组样品提取的样品量。结果:在提取方法相同且药材量相当的条件下,天王补心丸全方组提取得到的样品量明显高于缺桔梗组;桔梗+酸枣仁组提取得到的样品量明显高于桔梗组、酸枣仁组单煎得到的样品量之和;得到各组分离度较好的HPLC-PDA(Max Plot)和HPLC-ELSD 2种指纹图谱,且2者具有良好的相关性和互补性。结论:桔梗可以增加天王补心丸及主要药味酸枣仁水煎提取得到的样品量,可以增加其中某些成分的溶出及含量。
Objective:To establish the fingerprints of the active fractions from Tian-wang-bu-xin-wan decoction by HPLC, and to explain the role of Radix Platycodi in the decoction prescription.Method:The experimental conditions of the HPLC method were established as follows:Hanbon Lichrospher C_(18) column (4.6 mm×250 mm,5μm),mobile phase were methanol and 0.012 5 mol·L~(-1) ammonium acetate/acetic acid buffer,eluted with a linear gradient,flow rate was 1.0 mL·min~(-1),the photodiode array detector (PDA) and evaporative light scattering detector (ELSD) were connected in series.Result:In the same conditions and used same method,the extract amount of whole prescription was higher than that of excepting Radix Platycodi.The method provided two kinds of fingerprints with satisfied separation,which were the HPLC-PDA (Max Plot) fingerprint and the HPLC-ELSD fingerprint.And, they had good correlation and complementaritiy.Conclusion:The Radix Platycodi can enhance the decoction yield of the prescription;and increase the dissolution and the contents of some ingredients in the decoction.

关键词(KeyWords): 天王补心丸;桔梗;舟楫作用;高效液相色谱法;指纹图谱
Tianwang Buxinwan;Radix Platycodi;boat and oar role;high performance liquid chromatography;fingerprints

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基金项目(Foundation): 国家自然科学基金项目(30472202)

作者(Author): 刘萍;邹鹏;李外;孙艳;张洁;余河水;赵阳;马百平;
LIU Ping~1 ZOU Peng~2 LI Wai~1 SUN Yan~1 ZHANG jie~2 YU Heshui~2 ZHAO Yang~2 MA Baiping~(2*) (1. Pharmacy of Traditional Chinese Medicine, PLA General Hospital, Beijing 100853, China 2. Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China)

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