中国中药杂志

2013, v.38(01) 70-74

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芫花致肝毒性部位筛选及其肝毒性部位UPLC指纹图谱研究
Screening of hepatotoxicity fraction of Genkwa Flos and study on UPLC fingerprint of hepatotoxicity fraction

袁杨;耿璐璐;庄贺飞;孟夏;彭缨;毕开顺;陈晓辉;
YUAN Yang,GENG Lu-lu,ZHUANG He-fei,MENG Xia,PENG Ying,BI Kai-shun,CHEN Xiao-hui*(School of Pharmacy,Shenyang Pharmaceutical University,Shenyang 110016,China)

摘要(Abstract):

目的:确定芫花药材致肝毒性部位,为全面反映并控制其毒性部位的质量建立该部位超高效液相色谱指纹图谱。方法:通过血清生化指标和组织病理学筛选芫花致肝毒性部位;并以采用ACQUITY UPLC BEH C18色谱柱(2.1 mm×50mm,1.7μm),乙腈-0.05%磷酸溶液为流动相,梯度洗脱,210 nm为检测波长建立该部位UPLC指纹图谱。结果:确定了芫花致肝毒性部位为其乙醇提取物的氯仿萃取物;并建立了该部位的UPLC指纹图谱共有模式,标定了17个共有峰,指认了8个色谱峰,14批芫花药材致肝毒性部位的相似度为0.890~0.999。结论:芫花致肝毒性部位为其乙醇提取物的氯仿萃取物,所建立的UPLC指纹图谱,可为芫花临床安全用药提供依据。
Objective: To look for the active fraction of ethanol extract of Genkwa Flos(EGF) induced hepatotoxicity and develop an UPLC fingerprint of the active fraction.Method: Target fraction of EGF induced hepatotoxicity was guided by the serum biochemical and histopathology methods.The UPLC method was applied to establish the chromatographic fingerprint.The separation was achieved on a BEH C18 column(2.1 mm×50 mm,1.7 μm) with a mobile phase consisting of acetonitrile and water containing 0.05% phosphate acid running gradient elution.The detection was carried out at 210 nm and the analysis was finished within 10 min.Result: The chloroform phase of EGF could be responsible for the hepatotoxicity of this herb.The common mode of the UPLC fingerprint was set up under the established condition.There were 17 common peaks in fourteen batches of herbs,eight of which were identified,and the similar degrees of the fourteen batches to the common mode were between 0.890-0.999.Conclusion: It is easy to locate the chloroform extraction of EGF with hepatotoxicity.And the UPLC fingerprint was developed for the above fraction,which could provide valuable references for safe and effective clinical use of EGF.

关键词(KeyWords): 芫花;致肝毒性部位;指纹图谱;UPLC
Genkwa Flos;hepatotoxicity fraction;fingerprint;UPLC

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基金项目(Foundation): 国家“重大新药创制”科技重大专项(2009ZX09301-012)

作者(Author): 袁杨;耿璐璐;庄贺飞;孟夏;彭缨;毕开顺;陈晓辉;
YUAN Yang,GENG Lu-lu,ZHUANG He-fei,MENG Xia,PENG Ying,BI Kai-shun,CHEN Xiao-hui*(School of Pharmacy,Shenyang Pharmaceutical University,Shenyang 110016,China)

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