中国中药杂志

2013, v.38(19) 3313-3318

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盾叶薯蓣药材甾体总皂苷成分HPLC-ELSD指纹图谱的研究
Study on HPLC-ELSD fingerprint of total steroid saponins in herbs of Dioscorea zingiberensis

张新新;梁晋如;赵晔;孙文基;
ZHANG Xin-xin;LIANG Jin-ru;ZHAO Ye;SUN Wen-ji;Shaanxi Provincial Key Laboratory for Biological Medicine,Northwest University;

摘要(Abstract):

建立不同产地盾叶薯蓣药材中甾体总皂苷成分HPLC-ELSD指纹图谱的方法。采用Welchrom C18(4.6 mm×250 mm,5μm)色谱柱,乙腈(A)-水(B)作为流动相,梯度洗脱;柱温室温;体积流速1.0 mL·min-1;蒸发光散射检测器条件:漂移管温度90.0℃,载气(N2)流速2.8 L·min-1;进样体积10μL。通过检测10个批次盾叶薯蓣药材,首次建立了盾叶薯蓣甾体总皂苷成分HPLC-ELSD指纹图谱的共有模式,确定了25共有峰,并利用对照品对照法对其中的10个共有峰进行了成分指认。以共有模式对10批药材进行相似度评价,其相似度全部大于0.80。该方法准确、可靠、重复性好,为全面评价和控制盾叶薯蓣药材的质量提供了科学依据。
To establish a HPLC-ELSD fingerprint for total steroid saponins in herbs of Dioscorea zingiberensis. Welchrom C18( 4. 6 mm ×250 mm,5 μm) chromatographic column was adopted and eluted with the mobile phase of acetonitrile( A)-water( B) at the flow rate of 1. 0 mL·min- 1. The column temperature was room temperature. The ELSD conditions were as follows: the temperature of drift tube was 90. 0 ℃,the flow rate of carrier gas( N2) was 2. 8 L·min- 1,and the injection volume was 10 μL. After the detection of 10 batches of samples,the common mode of HPLC-ELSD fingerprint for total steroid saponins in herbs of D. zingiberensis was established for the first time,and 25 common peaks were determined. Among them,10 peaks were identified by comparing with reference substances. The similarities of 10 batches of herbs were evaluated in the common mode. All of them were higher than 0. 80. This method is so accurate,reliable and highly repeatable that it can provide scientific basis for evaluating and controlling the quality of total steroid saponins in herbs of D. zingiberensis.

关键词(KeyWords): 盾叶薯蓣;甾体总皂苷;HPLC-ELSD;指纹图谱
Dioscorea zingiberensis;total steroid saponins;HPLC-ELSD;fingerprint

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作者(Author): 张新新;梁晋如;赵晔;孙文基;
ZHANG Xin-xin;LIANG Jin-ru;ZHAO Ye;SUN Wen-ji;Shaanxi Provincial Key Laboratory for Biological Medicine,Northwest University;

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