中国中药杂志

2011, v.36(11) 1471-1473

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土鳖虫药材高效毛细管电泳指纹图谱鉴别研究
Research on HPCE fingerprint of Eupolyphaga Steleophaga

李峰;王成芳;包永睿;
LI Feng,WANG Chengfang,BAO Yongrui (College of Pharmacy Department,Liaoning University of Traditional Chinese Medicine,Dalian,116600,China)

摘要(Abstract):

目的:建立土鳖虫药材HPCE指纹图谱鉴别方法。方法:采用高效毛细管电泳法,未涂层石英毛细管柱(40 cm×75μm),20 mmol.L-1硼砂(pH 9.44)缓冲液、柱温25℃、分离电压13 kV、检测波长265 nm。结果:通过中药色指纹图谱相似度评价系统,确定6个共有峰构成土鳖虫药材指纹图谱的特征峰。结论:本方法为土鳖虫药材品种鉴定与质量控制提供了依据。
Objective:To set up the fingerprint of HPCE for medicinal material Eupolyphaga Steleophaga.Method:Separation was performed at 25 ℃ on an Agilent uncoated silica capillary column(40 cm×75 μm) with 20 mmol·L-1 borax buffer solution(pH 9.44) as CE buffer.The isolating voltage was 13 KV,and the DAD detection was set at 265 nm.Result:The characteristic peak of fingerprint of Eupolyphaga Steleophaga was consisted of 6 common peaks.Conclusion:The method can be used for the quality control of Eupolyphaga Steleophaga.

关键词(KeyWords): 土鳖虫;高效毛细管电泳;指纹图谱
Eupolyphaga Steleophaga;HPCE;fingerprint

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基金项目(Foundation): 国家自然科学基金项目(30973881);; 辽宁省教育厅项目(20060547);; 沈阳市科技局项目(1081238-1-00)

作者(Author): 李峰;王成芳;包永睿;
LI Feng,WANG Chengfang,BAO Yongrui (College of Pharmacy Department,Liaoning University of Traditional Chinese Medicine,Dalian,116600,China)

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