中国中药杂志

2019, v.44(15) 3343-3348

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疏肝和胃汤及其有效物质部位对抑郁模型大鼠下丘脑内多种神经递质含量的影响
Effects of Shugan Hewei Decoction and active substance fractions on behavior and neurotransmitter levels in hypothalamus of depression model rats

牟雄军;刘昊;林宁;陈新;刘松林;
MOU Xiong-jun;LIU Hao;LIN Ning;CHEN Xin;LIU Song-lin;Hubei Key Laboratory of Resource and Chemistry of Chinese Medicine,Hubei University of Chinese Medicine;

摘要(Abstract):

研究疏肝和胃汤及其有效物质部位对抑郁模型大鼠行为学及下丘脑内神经递质含量的影响并初步探讨其可能的作用机制。雄性SD大鼠随机分为空白对照组,模型组,阳性对照氟西汀组,疏肝和胃汤全方高、低剂量组,3组不同有效物质部位高、低剂量组。运用CUMS结合孤养制作抑郁模型,造模3周后灌胃治疗7 d。采用行为学(糖水消耗实验、旷场实验、强迫游泳实验)评价大鼠抑郁状态,并通过LC-MS/MS测定下丘脑内多巴胺(DA)、去甲肾上腺素(NE)、5-羟色胺(5-HT)、5-吲哚乙酸(5-HIAA)、γ-氨基丁酸(GABA)、谷氨酸(Glu)含量。结果与正常对照组比较,模型组大鼠糖水消耗减少(P<0. 01);旷场运动距离和穿越中央区域次数明显减少(P<0. 01,P<0. 01),静止时间显著增加(P<0. 01);强迫游泳不动时间显著延长(P<0. 01);下丘脑内DA,5-HT,NE,5-HIAA,GABA水平显著降低(P<0. 01),Glu水平显著增加(P<0. 01);与模型组比较,氟西汀组,疏肝和胃汤全方组,挥发油类组,总多糖类组和萜类物质组的上述检测指标均改变(P<0. 05或P<0. 01)。疏肝和胃汤全方及其有效物质部位能改善抑郁模型大鼠行为学各指标,且可能通过调节下丘脑神经递质含量而缓解抑郁症状。
To investigate the effects of Shugan Hewei Decoction and its active substance fractions on behavior and neurotransmitter levels in hypothalamus of depression model rats,and preliminarily explore its possible mechanism. Male SD rats were randomly divided into blank control group,model group,fluoxetine( positive control) group,Shugan Hewei Decoction high and low dose groups,high and low dose groups of three different substance fractions. After 3 weeks' CUMS and social isolation to induce models,intragastrical administration lasted for 7 d. Behavioral experiments( sucrose consumption test,open-field test,and forced swimming test) were then performed to evaluate the depression status of rats. Several neurotransmitters in hypothalamus of rats were determined by LC-MS/MS method,including dapamine( DA),norepinephrine( NE),serotonin( 5-HT),5-indoleacetic acid( 5-HIAA),γ-aminobutyric acid( GABA),and glutamic acid( Glu). As compared with the blank control group,the sucrose consumption was reduced( P<0. 01); the total distance and the number of crossing the central area were also significantly reduced( P< 0. 01,P< 0. 01),while the resting time increased significantly( P<0. 01); the forced swimming time was significantly prolonged( P<0. 01); DA,5-HT,NE,5-HIAA and GABA levels in hypothalamus were significantly reduced( P < 0. 01),while Glue level was significantly increased( P < 0. 01) in model group. As compared with the model group,all the above indexes had changes in fluoxetine group,Shugan Hewei Decoction whole recipe groups,volatile oils group,polysaccharides group,and terpenoids group( P<0. 01 or P<0. 05). Shugan Hewei Decoction whole recipe and its active substance fractions can improve the behavior of depression model rats and may exert anti-depression effects by regulating the content of neurotransmitters in the hypothalamus.

关键词(KeyWords): 疏肝和胃汤;行为学;下丘脑;神经递质;有效物质部位;抑郁模型大鼠
Shugan Hewei Decoction;behavior;hypothalamus;neurotransmitters;active substance fractions;depression model rats

Abstract:

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基金项目(Foundation): 国家自然科学基金项目(81573960);; 湖北省教育厅科学技术研究项目(Q20182009);; 湖北省高等学校优秀中青年科技创新团队计划项目(T201808)

作者(Author): 牟雄军;刘昊;林宁;陈新;刘松林;
MOU Xiong-jun;LIU Hao;LIN Ning;CHEN Xin;LIU Song-lin;Hubei Key Laboratory of Resource and Chemistry of Chinese Medicine,Hubei University of Chinese Medicine;

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