中国中药杂志

2021, v.46(20) 5240-5246

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一株玉米赤霉烯酮高效降解细菌的筛选与降解条件研究
Screening of zearalenone-degrading bacteria and analysis of degradation conditions

邓桃;袁青松;周涛;郭兰萍;江维克;周世华;杨昌贵;康传志;
DENG Tao;YUAN Qing-song;ZHOU Tao;GUO Lan-ping;JIANG Wei-ke;ZHOU Shi-hua;YANG Chang-gui;KANG Chuan-zhi;Guizhou University of Traditional Chinese Medicine;National Resource Center for Chinese Meteria Medica, China Academy of Chinese Medical Sciences;

摘要(Abstract):

玉米赤霉烯酮毒素(zearalenone, ZEN)是由镰刀菌属真菌产生的一种真菌毒素,具有雌激素样效应、致癌性及多种毒性。为寻求更高效、实用的生物脱毒制剂,提高其应用范围。该研究从发病霉变太子参块根中分离获得194株细菌菌株,将分离菌株与ZEN毒素共培养,HPLC检测筛选出1株ZEN高效降解细菌,编号为H4-3-C1,形态观察及分子鉴定为醋酸钙不动杆菌Acinetobacter calcoaceticus。分别研究了培养基、接种量、培养时间、培养基pH、温度等对H4-3-C1菌株降解ZEN的影响;同时,初步探讨该菌株清除ZEN的机制及其对薏苡仁中ZEN的降解效果。H4-3-C1菌株在pH 6的LB培养基、1%的接种量、28℃、180 r·min~(-1)的条件下振荡培养24 h后,对5μg·mL~(-1 )ZEN的降解率最高可达85.77%;菌株培养上清液对ZEN的降解率远高于菌体胞内液和菌体,由此推测该菌株主要通过分泌胞外酶降解ZEN;且H4-3-C1菌株也能很好地降解中药材薏苡仁中的ZEN,可以将薏苡仁中ZEN初始量由90μg·g~(-1)降解至40.68μg·g~(-1),降解率达54.80%。该研究为食品加工产物及中药材中ZEN的生物脱毒提供了新的菌株资源和应用技术。
Zearalenone(ZEN) is a mycotoxin produced by Fusarium, possessing estrogen-like effects, carcinogenicity, and multiple toxicities. To seek more efficient and practical agents for biological detoxification and broaden their application, this study isolated 194 bacterial strains from the moldy tuberous root of Pseudostellaria heterophylla, which were co-cultured with ZEN. An efficient ZEN-degrading strain H4-3-C1 was screened out by HPLC and identified as Acinetobacter calcoaceticus by morphological observation and molecular identification. The effects of culture medium, inoculation dose, culture time, pH, and temperature on the degradation of ZEN by H4-3-C1 strain were investigated. The mechanism of ZEN degradation and the degrading effect in Coicis Semen were discussed. The degradation rate of 5 μg·mL~(-1) ZEN by H4-3-C1 strain was 85.77% in the LB medium(pH 6) at 28 ℃/180 r·min~(-1) for 24 h with the inoculation dose of 1%. The degradation rate of ZEN in the supernatant of strain culture was higher than that in the intracellular fluid and thalli. The strain was inferred to secret extracellular enzymes to degrade ZEN. In addition, the H4-3-C1 strain could also degrade ZEN in Coicis Semen. If the initial content of ZEN in Coicis Semen was reduced from 90 μg·g~(-1) to 40.68 μg·g~(-1), the degradation rate could reach 54.80%. This study is expected to provide a new strain and application technology for the biological detoxification of ZEN in food processing products and Chinese medicinal materials.

关键词(KeyWords): 玉米赤霉烯酮;生物降解;不动杆菌;降解特性
zearalenone(ZEN);biodegradation;Acinetobacter calcoaceticus;degradation

Abstract:

Keywords:

基金项目(Foundation): 中央本级重大增减支项目(2060302);; 现代农业产业技术体系建设专项(CARS-21);; 贵州省高层次创新型人才项目(黔科合平台人才[2018]5638-2);; 贵州省科技创新人才团队项目(黔科合平台人才[2019]5611);; 贵州省科技计划项目(黔科合支撑[2021]一般418)

作者(Author): 邓桃;袁青松;周涛;郭兰萍;江维克;周世华;杨昌贵;康传志;
DENG Tao;YUAN Qing-song;ZHOU Tao;GUO Lan-ping;JIANG Wei-ke;ZHOU Shi-hua;YANG Chang-gui;KANG Chuan-zhi;Guizhou University of Traditional Chinese Medicine;National Resource Center for Chinese Meteria Medica, China Academy of Chinese Medical Sciences;

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