中国中药杂志

2015, v.40(12) 2325-2329

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基于肝细胞毒价检测的何首乌炮制工艺比较研究
Comparative study on preparation of Polygoni Multiflori Radix based on hepatotoxic bioassay

马致洁;李晓菲;吕旸;蒋冰倩;赵艳玲;张雅铭;赵奎君;王伽伯;肖小河;
MA Zhi-jie;LI Xiao-fei;LV Yang;JIANG Bing-qian;ZHAO Yan-ling;ZHANG Ya-ming;ZHAO Kui-jun;WANG Jia-bo;XIAO Xiao-he;Beijing Friendship Hospital,Capital Medical University;Department of Pharmacy,302 Military Hospital;China Military Institute of Chinese Medicine,302 Military Hospital;

摘要(Abstract):

采用肝细胞毒价检测方法评价何首乌不同炮制品的毒性,优选炮制工艺。以同一批生何首乌为原料,分别采用高压清蒸、高压黑豆汁蒸、常压清蒸法炮制何首乌,以正常人肝细胞(L02)为模型,细胞毒价为指标,评价不同蒸制方法、蒸制时间的何首乌炮制品肝细胞毒性,并对部分炮制品进行UPLC-MS分析。结果显示,肝细胞毒价检测方法能有效评价何首乌不同炮制品的毒性,不同炮制方法均可减轻何首乌的毒性,高压清蒸3 h减毒效果较佳,不同炮制方法对何首乌化学成分的影响不同,3种方法炮制品与生品比较,没食子酸、二苯乙烯苷、大黄素8-O-β葡萄糖苷、大黄素均有明显降低,其中二苯乙烯苷含量与其毒价变化趋势基本一致。由此可知,何首乌经炮制可减毒,炮制方法、时间对何首乌成分及肝毒性均有影响,且高压清蒸3 h减毒效果较佳,建议进一步加强何首乌炮制减毒控制标准研究。
Toxicity of different processed was evaluated Polygoni Multiflori Radix by determining the hepatotoxic potency for selecting processing technology. Process Polygoni Multiflori Radix using high pressure steamed,Black Bean high pressure steamed,atmospheric steamed for different time. Using normal human hepatocytes( L02) as evaluation model,hepatotoxic potency as index to evaluate hepatotoxic potency of different processed Polygoni Multiflori Radix. Analysis chemical composition of some processed products by UPLC-MS. Hepatotoxic bioassay method cloud evaluate the toxicity of different Polygoni Multiflori Radix samples. Different processing methods can reduce the toxicity of Polygoni Multiflori Radix,high pressure steamed three hours attenuated was better. Different processing methods have different effects on chemical constituents of Polygoni Multiflori Radix. Comparing with crude sample,the contents of gallic acid,2,3,5,4-tetrahydroxyl diphenylethylene-2-O-glucoside,emodin-8-O-beta glucoside and emodin were decreased in processed products with 3 kinds of different methods. The change trend of 2,3,5,4-tetrahydroxyl diphenylethylene-2-O-glucoside content was similar with hepatotoxic potency. Different processing methods can reduce the toxicity of Polygoni Multiflori Radix. Processing methods and time attenuated obvious impact on toxicity. Recommended further research on the attenuated standard control of Polygoni Multiflori Radix concocted.

关键词(KeyWords): 何首乌;肝毒性;炮制减毒;毒价检测;L02细胞
Polygoni Multiflori Radix;hepatotoxicity;processing attenuated;toxic potency;L02 cells

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金面上项目(81373984);; 中央本级课题(20603020101);; 首都医科大学中医药专项(14ZY12);; 国家“重大新药创制”科技重大专项(2015ZX09501-004-001-008);; 国家公益性行业科研专项(201507004-04)

作者(Author): 马致洁;李晓菲;吕旸;蒋冰倩;赵艳玲;张雅铭;赵奎君;王伽伯;肖小河;
MA Zhi-jie;LI Xiao-fei;LV Yang;JIANG Bing-qian;ZHAO Yan-ling;ZHANG Ya-ming;ZHAO Kui-jun;WANG Jia-bo;XIAO Xiao-he;Beijing Friendship Hospital,Capital Medical University;Department of Pharmacy,302 Military Hospital;China Military Institute of Chinese Medicine,302 Military Hospital;

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