中国中药杂志

2010, v.35(03) 348-351

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丹参酚酸A对大鼠肝微粒体细胞色素P450酶系的影响
Effects of salvianolic acid A on rat liver microsomal cytochrome P450 system

郭海方;邹晓丽;许卉;刘珂;
GUO Haifang1,ZOU Xiaoli1,XU Hui1,LIU Ke1,2 (1. School of Pharmacy,Yantai University,Yantai 264005,China; 2.Shandong Target Drug Research Co.Ltd.,Yantai 264005,China)

摘要(Abstract):

目的:研究丹参酚酸A对大鼠肝微粒体细胞色素P450和细胞色素b5含量以及CYP1A2和CYP2E1活性的影响。方法:将大鼠分成溶剂对照组和丹参酚酸A给药组,每组10只,雌雄各半,丹参酚酸A给药组尾静脉注射给予丹参酚酸A20mg·kg-1.d-1,连续给药5d;溶剂对照组给予相同剂量的溶剂,紫外分光光度法测定大鼠肝微粒体细胞色素P450和细胞色素b5含量;探针底物法评价CYP1A2和CYP2E1的活性。结果:丹参酚酸A尾静脉注射连续给药5d后,大鼠细胞色素P450和细胞色素b5含量与对照组比较均无显著性差异;CYP1A2和CYP2E1的活性与对照组比较也无显著性差异。结论:丹参酚酸A对CYP1A2和CYP2E1没有诱导或抑制作用,与经过CYP1A2和CYP2E1代谢的药物发生相互作用的可能性较小。
Objective:To study the effects of salvianolic acid A on content of cytochrome P450,cytochrome b5 and CYP1A2,CYP2E1 activities of rats. Method:The rats were randomly divided into two groups and each group contained 5 male rats and 5 female rats. One is control group,another is dosage group. The dosage group was injected salvianolic acid A into a rat tail vein at doses of 20 mg·kg-1·d-1 for 5 days. The control group was injected placebo into a rat tail vein at the same doses as the dosage group. The content of cytochrome P450 and cytochrome b5 of rats were assayed using UV and CYP1A2,CYP2E1 activities were evaluated using probe substrate. Result:After salvianolic acid A was injected into rats tail vein for 5 days,the total content of cytochrome P450 and cytochrome b5 and CYP1A2 and CYP2E1 activities have no statistical significance of differences than the control group. Conclusion:Salvianolic acid A has no effects on CYP1A2 and CYP2E1 activities,indicating that there is no internation between salvianolic acid A and the drugs metabolized by CYP1A2 or CYP2E1.

关键词(KeyWords): 丹参酚酸A;细胞色素P450;CYP1A2;CYP2E1;细胞色素b5
salvianolic acid A (Sal A); cytochrome P450 (CYP450); CYP1A2; CYP2E1; cytochrome b5

Abstract:

Keywords:

基金项目(Foundation): 山东省高等学校科技计划项目(J09LF08);; 山东省高校优秀青年教师国内访问学者项目(2007)

作者(Authors): 郭海方;邹晓丽;许卉;刘珂;
GUO Haifang1,ZOU Xiaoli1,XU Hui1,LIU Ke1,2 (1. School of Pharmacy,Yantai University,Yantai 264005,China; 2.Shandong Target Drug Research Co.Ltd.,Yantai 264005,China)

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