中国中药杂志

2020, v.45(14) 3387-3394

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模式生物斑马鱼在中药药效物质多模态辨识中的应用
Application of zebrafish model in multimodal identification of active constituents of traditional Chinese medicine

陈静;赵璐;王毅;
CHEN Jing;ZHAO Lu;WANG Yi;Department of Chinese Medicine Science & Engineering, College of Pharmaceutical Sciences, Zhejiang University;

摘要(Abstract):

中药药效物质研究亟需合适的高通量整体动物模型以体现其整合调节作用。斑马鱼模式生物具备体型小、繁殖能力强、体外发育等独特优势,且其遗传生物特性与人类高度保守,是研究人体多系统病理过程的理想疾病模型,在中药药效物质研究领域也有越来越多的研究应用。该文对近年来发表的使用斑马鱼模型进行中药药理效应多模态研究的相关文章进行回顾,总结了斑马鱼作为动物模型,在血管新生、炎症、神经行为学病变、心脏损伤、骨质疏松、高血糖、高血脂及肿瘤等疾病中的应用,阐述了斑马鱼模型在中药多模态研究中的优势与主要发现,展望了运用免疫组化、荧光探针及转基因斑马鱼品系,在活体动物中动态观测并定量分析目标器官或细胞的变化,从而实现中药药效物质多模态辨识,并进一步结合生物标记物表达水平的检测,探索中药药效物质机制。
Suitable animal models with high throughput are required to investigate the integrating and regulating effects of active constituents of traditional Chinese medicine(TCM). The zebrafish model has become an ideal research model for the diseases of various human systems due to its unique features, such as small body size, strong reproductive capacity, in vitro development, and high homology with human beings, and has been increasingly studied and applied in the field of TCM pharmacological effects. Here we reviewed recent published papers of zebrafish-based multimodal studies, focusing on the pharmacological effects of TCM. The applications of zebrafish model in studies of human diseases were systematically summarized, including angiogenesis, inflammation, neurobehavior changes, heart injury, osteoporosis, hyperglycemia, hyperlipidemia, and tumors. The advantages and major findings of zebrafish models in multimodal TCM studies were discussed. In addition, we anticipated the use of immunohistochemistry, fluorescent probes and transgenic fish lines to dynamically observe and quantitatively analyze the endogenous changes of target organs or cells in the zebrafish model, so as to realize the multimodal identification of active compositions of TCM, and further explore the mechanism of active compositions of TCM by considering the detected expression levels of biomarkers.

关键词(KeyWords): 斑马鱼;中药;药理效应;多模态研究
zebrafish;traditional Chinese medicine;pharmacological effects;multimodal research

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基金项目(Foundation): 国家自然科学基金项目(31971088,81822047)

作者(Author): 陈静;赵璐;王毅;
CHEN Jing;ZHAO Lu;WANG Yi;Department of Chinese Medicine Science & Engineering, College of Pharmaceutical Sciences, Zhejiang University;

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