中国中药杂志

2004, (01)

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鹿茸粗提液对大鼠成骨肉瘤细胞增殖的调节作用
The modulation of pilose antler extract (PAE) on the prolifera tion of rat osteogenic cells UMR-106

陈晓超,柯李晶,陈躬瑞,刘树滔,霍玉书,饶平凡
CHEN Xiao-chao 1, KE Li-jing 1, CHEN Gong-rui 1, LIU Shu-ta o 1, HUO Yu-shu 2, RAO Ping-fan 1 (1.Institute of Biotechnology, Fuzhou University, Fuzhou 350002, Ch ina; 2. Health Science Center at San Antonio, The University of Texas, Texas 78229-3 900,USA)

摘要(Abstract):

目的 :观察鹿茸粗提液 (PAE)对大鼠成骨肉瘤细胞系UMR 10 6增殖的影响 ,从而为研究鹿茸强筋健骨的分子机理以及筛选鹿茸防治骨质疏松的生物活性成分提供实验依据。方法 :通过SephacrylS 2 0 0HR凝胶过滤色谱对PAE进行初级分离 ,用MTT法检测不同分离组分对UMR 10 6细胞增殖的影响。结果 :发现SephacrylS 2 0 0HR凝胶过滤色谱第 2峰P2次组分 ,当其蛋白质浓度高于 0 .972mg·L - 1并低于 97.2mg·L - 1时 ,对UMR 10 6细胞增殖活性的影响表现为抑制 ;若浓度达到 97.2mg·L- 1后则会促进细胞的增殖 ,且增殖效应随蛋白质浓度的增加而明显上升。当样品蛋白质浓度达到 9.72g·L- 1时 ,相应的细胞增殖率为 477.92 % ,接近PAE的增殖水平 (499.62 % )。经SDS PAGE电泳分析第 2峰分子量集中于 59kDa左右。此外 ,发现第 3、第 4和第 5峰对UMR 10 6细胞增殖有显著的抑制作用。结论 :鹿茸蛋白中含有多种双向调控UMR 10 6细胞增殖的生物活性因子 ,既有剂量双向效应 ,也有与分子量相关的不同调控蛋白 ,这对中药的双向调节作用提供研究线索
Objective: To investigate the modulation o f pilose antler extract (PAE) on rat osteo genic cells UMR-106 in vitro. Method: Component P2 of PAE was isolated by Sephacryl S-200HR gel filtration chromatogr aphy. The proliferative effects of P2 and other components isolated by Sephacryl S-200HR on UMR-106 cells were investigated by MTT assay. Result: The P2 could significantly increase the proliferation rate of osteoge nic cells. When the protein concentration of P2 was between 0.972 mg·L -1 and 97.2 mg·L -1 , it could inhibit the proliferation of UMR-106 cells. Bu t while the concentration was equal to or greater than 97.2 mg·L -1 , the P 2 could increase the proliferation rate of cells, especially 477.92% at 9.72 g· L -1 , which was approximated to 499.62% of PAE. The molecular weight of the P2 was about 59kDa determined by SDS-PAGE. On the other hand, inhibition was also observed in the sample of the P3, P4 and P5. Conclusion: Those regulative factors in PAE which have different molecular weight c an affect the proliferation of UMR-106 cells two-wayly. And this adjustment al so relies on the dose of those factors. This finding may help us to understand t he possible mechanism of Chinese traditional medicine from animal materials.

关键词(KeyWords): 鹿茸粗提液(PAE);UMR106细胞;细胞增殖;MTT法
pilose antler extract (PAE); UMR-106 cell s; cell proliferation; MTT assay

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作者(Author): 陈晓超,柯李晶,陈躬瑞,刘树滔,霍玉书,饶平凡
CHEN Xiao-chao 1, KE Li-jing 1, CHEN Gong-rui 1, LIU Shu-ta o 1, HUO Yu-shu 2, RAO Ping-fan 1 (1.Institute of Biotechnology, Fuzhou University, Fuzhou 350002, Ch ina; 2. Health Science Center at San Antonio, The University of Texas, Texas 78229-3 900,USA)

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