中国中药杂志

2014, v.39(15) 2814-2818

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不同加工方法对天麻有效成分及SO_2残留量的影响
Effects of different processing methods on effective components and sulfur dioxide residue in Gastrodiae Rhizoma

宁子琬;毛春芹;陆兔林;季德;刘静;季琳;杨欢;王法琴;
NING Zi-wan;MAO Chun-qin;LU Tu-lin;JI De;LIU Jing;JI Lin;YANG Huan;WANG Fa-qin;College of Pharmacy,Nanjing University of Chinese Medicine;Key Research Laboratory of Chinese Medicine Processing of Jiangsu Province;

摘要(Abstract):

比较不同加工方法对天麻中有效成分含量及SO2残留量的影响。采用HPLC测定天麻中5种有效成分的含量,Merck Purospher STAR色谱柱(4.6 mm×250 mm,5μm);甲醇(A)-0.1%甲酸水(B)为流动相,梯度洗脱;流速1 mL·min-1;柱温为35℃;检测波长为270 nm;进样量为10μL。在上述色谱条件下,5种成分均得到很好的分离,各成分的质量浓度与峰面积之间线性关系良好(r≥0.999 8),平均回收率为98.38%~102.5%(RSD≤1.7%)。天麻药材经煮制、硫磺熏蒸后,腺苷、天麻素、对羟基苯甲醇、对羟基苯甲醛、巴利森苷的含量与蒸制所得天麻药材相比均有不同程度的降低;且经硫磺熏蒸后的天麻药材二氧化硫残留量均超过400 mg·kg-1,此为制定天麻药材加工干燥的质量标准提供了科学依据。
The contents of adenosine,gastrodin,4-hydroxybenzyl alcohol,4-hydroxybenzaldehyde,parishin and sulfur dioxide residue were compared in differently-processed Gastrodiae Rhizoma to provide the basis for a reasonable processing method of Gastrodiae Rhizoma.The analysis was performed on a Merck Purospher STAR column(4.6 mm × 250 mm,5 μm) with a mobile phase consisting of methanol and water(containing 0.1% formic acid) under gradient elution at a flow rate of 1.0 mL · min- 1.The eluates were detected at 270 nm,and the column temperature was 35 ℃.The content of adenosin,gastrodin,4-hydroxybenzyl alcohol,4-hydroxybenzaldehyde and parishin in processing of boiling or sulfur-fumigated were lower than that of in processing of steaming.Furthermore,the sulfur dioxide residue of sulphur-fumigated groups exceed 400 mg·kg- 1.This stable and reliable method will contribute to the quality control of different processed Gastrodiae Rhizoma.

关键词(KeyWords): 天麻;蒸煮工艺;硫磺熏蒸;HPLC;有效成分
Gastrodiae Rhizoma;processing methods;sulfur-fumigated;HPLC;effective component

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基金项目(Foundation): 科技部行业专项(201307008)

作者(Author): 宁子琬;毛春芹;陆兔林;季德;刘静;季琳;杨欢;王法琴;
NING Zi-wan;MAO Chun-qin;LU Tu-lin;JI De;LIU Jing;JI Lin;YANG Huan;WANG Fa-qin;College of Pharmacy,Nanjing University of Chinese Medicine;Key Research Laboratory of Chinese Medicine Processing of Jiangsu Province;

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