中国中药杂志

2015, v.40(03) 480-483

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羊耳菊药材的HPLC指纹图谱研究
Researching on fingerprint of Inulacappa by HPLC

熊荻菲菲;朱迪;谭丹;兰燕宇;郑林;王爱民;
XIONG Di-fei-fei;ZHU Di;TAN Dan;LAN Yan-yu;ZHENG Lin;WANG Ai-min;Guizhou Provincial Key Laboratory of Pharmaceutics,School of Pharmacy,Guiyang Medical College;Engineering Research Center for the Development and Application of Ethnic Medicine and Traditional Chinese Medicine( Ministry of Education) ,Guiyang Medical College;

摘要(Abstract):

目的:建立羊耳菊药材的高效液相指纹图谱,并对共有色谱峰进行指认,为其质量控制方法提出科学依据。方法:采用HPLC-DAD检测法,Agilent Eclipse Plus C18柱(2.1 mm×150 mm,1.8μm);流动相0.1%甲酸水-0.1%甲酸乙腈溶液梯度洗脱;柱温45℃;检测波长325 nm;流速0.3 m L·min-1。结果:对10批羊耳菊药材进行测定,标定了17个共有峰,指认了其中5个共有峰,10批羊耳菊药材的相似度均在0.95以上。结论:该方法可靠、简便,可用于羊耳菊药材的质量评价。
Objective: This study is to establish the fingerprint and find out the common chromatographic peaks of Inula cappa by HPLC. Method: The HPLC analysis was performed onan Agilent Eclipse Plus C18 column( 2. 1 mm × 150 mm,1. 8 μm) with 0. 1% fomic acid aqueous solution-0. 1% fomic acid acetonitrile solution as mobile phase at a flow rate of 0. 3 mL ·min- 1; The detective wavelength is 325 nm; The column temperature is 45 ℃. Result: The results indicated that 5 of 17 common peaks were identified. The similarity about 10 groups of Inulacappais is over 0. 95. Conclusion: This method is able to be a scientific basis of quality assessment according to its convenient and reliable.

关键词(KeyWords): 羊耳菊药材;HPLC-DAD;指纹图谱
Herbs of Inula cappa;HPLC-DAD;fingerprint

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(81360680);; 国家科技支撑计划课题项目(2013BAI11B01);; 贵州省中药现代化科技产业研究开发专项(黔科合重G字[2013]4001)

作者(Author): 熊荻菲菲;朱迪;谭丹;兰燕宇;郑林;王爱民;
XIONG Di-fei-fei;ZHU Di;TAN Dan;LAN Yan-yu;ZHENG Lin;WANG Ai-min;Guizhou Provincial Key Laboratory of Pharmaceutics,School of Pharmacy,Guiyang Medical College;Engineering Research Center for the Development and Application of Ethnic Medicine and Traditional Chinese Medicine( Ministry of Education) ,Guiyang Medical College;

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