中国中药杂志

2004, (08)

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肾乐胶囊对大鼠5/6肾切除肾衰模型作用机理的实验研究
Curative machanism of Shenle capsule on 5/6 nephrectomy rats

魏日胞,张五星,陈香美
WEI Ri-bao, ZHANG Wu-xing, CHEN Xiang-mei(Division of Nephrology, Kidney Center & Key Lab of Chinese PLA, General Hospital of Chinese PLA, Beijing 100853, China)

摘要(Abstract):

目的 :通过对大鼠 5 / 6肾切除 (5 / 6NX)所致肾衰模型的研究 ,探讨中药复方肾乐胶囊治疗慢性肾衰的作用机理。方法 :采用纤维蛋白原平板溶环法 ,检测尿中纤溶酶原激活物PA的活性 ;用半定量法分析残肾组织中组织型纤溶酶原激活物 ,尿激酶型纤溶酶原激活物 /纤溶酶原激活物抑制物 (tPA ,uPA/PAI 1)的染色强度和面积 ;用Northernblot来观察转化生长因子β(TGF β)mRNA的表达。 结果 :模型对照组大鼠尿PA活性下降 ,肾组织tPA ,uPA表达下降 ;而肾组织中PAI 1和TGF βmRNA表达增高。肾乐胶囊治疗后 ,尿PA的活性升高 ,肾组织中tPA ,uPA的表达增强 ;而肾组织中PAI 1和TGF βmRNA的表达却下降。 结论 :肾乐胶囊可以通过调节 5 / 6NX模型大鼠尿PA的活性 ,肾组织tPA ,uPA/PAI 1,TGF βmRNA的表达 ,延缓肾小球硬化和间质纤维化病变的进展
Objective: To explore curative machanism of Shenle capsule on the 5/6 nephrectomy rats. Method: Fibrin plate method was applied to examine activity of urinary plasminogen activator(PA). Semi-quantitative analysis was used to observe stained intensity and area of tissue-type plasminogen activator, urokinas-type plasminogen activator/ plasminogen activator inhibitor(tPA, uPA/PAI-1)in remnant renal tissue. Northern blot was employed to analyze the expression of transforming growth factor (TGF-β)mRNA. Result: In model control group,the urinary PA activity and protein expression of tPA, uPA were down-regulated, but protein expression of PAI-1, TGF-β mRNA was up-regulated in remnant renal tissue. In each treated group, the urinary PA activity and protein expression of tPA/uPA were enhanced,but the protein expression of PAI-1, TGF-βmRNA decreased simultaneously. Conclusion: Shenle capsule can delay glomerulosclersis and tubulointerstitial fibroticlesions of remnant kidney by improving the activity of urinary PA and modulating the expression of tPA,uPA/PAI-1 and TGF-βmRNA.

关键词(KeyWords): 肾乐胶囊;纤溶酶原激活物;纤溶酶原激活物Ⅰ型抑制物;转化生长因子β
Shenle capsule; plasminogen activator(PA); I-type plasminogen activator inhibitor(PAI-1); transforming growth factor(TGF-β)

Abstract:

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基金项目(Foundation): 国家“十五”科技攻关课题 ( 2 0 0 1BA70 1A14a)

作者(Author): 魏日胞,张五星,陈香美
WEI Ri-bao, ZHANG Wu-xing, CHEN Xiang-mei(Division of Nephrology, Kidney Center & Key Lab of Chinese PLA, General Hospital of Chinese PLA, Beijing 100853, China)

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