中国中药杂志

2004, (11)

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天麻钩藤方对自由活动大鼠脑缺血海马细胞外液递质氨基酸的影响
Effects of Tianma Gouteng Fang on transmitter amino acids in the hippocampus extracellular liquids in freely moving rats subjected to brain ischemia

张春颖,杜贵友,王巍,叶祖光,王丹巧,孙晓芳,赵德忠
ZHANG Chun-ying1, DU Gui-you1, WANG Wei2, YE Zu-guang3 , WANG Dan-qiao2, SUN Xiao-fang2, ZHAO De-zhong2 (1. Institute of Chinese Materia Medica, China Academy of Traditio nal Chinese Medicine, Beijing 100700, China; 2. Geriatrics Institute, Xiyuan Hospital, China Academy of Traditional Chines e Medicine, Beijing 100091, China; 3. State Food and Drug Administration, Beijing 100038, China) [

摘要(Abstract):

目的 :观察天麻钩藤方 (天钩方 )对不完全脑缺血清醒自由活动大鼠海马细胞外液递质氨基酸的影响。方法 :用CMA微透析采样系统收集双侧颈总动脉结扎和放松引起的清醒自由活动大鼠不完全缺血前、缺血期间及再灌后大鼠海马细胞外透析液 ;用高效液相色谱 电化学法测定透析液中谷氨酸 (Glu)、牛磺酸 (Tau)、精氨酸(Arg)、γ 氨基丁酸 (GABA)和半胱氨酸 (Cys)的浓度。结果 :天钩方中剂量组可升高正常大鼠脑海马细胞外液GABA和Tau的浓度。与模型组比较 ,大鼠不完全缺血 12 0min时 ,天钩方中和大剂量组的Glu水平分别降低了 38 6 4 %和31 35 % ,Tau升高了 13 99%和 12 86 % ,GABA升高了 2 5 89%和 33 99% ,Cys降低了 4 0 93%和 4 2 0 8% ,Arg升高了16 95 %和 8 96 % ;再灌 12 0min时 ,天钩方中和大剂量组的Glu的水平分别降低了 14 5 5 %和 11 4 8% ,Tau升高了16 13%和 14 0 3% ,GABA升高了 2 4 4 1%和 2 6 2 2 %。结论 :天钩方能增加正常大鼠海马细胞外液中抑制性氨基酸的浓度 ,抑制缺血再灌引起的兴奋性氨基酸的过度释放 ,增加Arg和抑制性氨基酸的浓度 ,从而发挥神经保护作用
Objective: To observe the effects of Tian ma Gouteng Fang (TGF) on the transmitter amino acids in the hippocampus extracellu lar liquids in freely moving rats subjected to incomplete brain ischemia. Method: Hippocampus extracellular liquids was collected conti nuously by the microdialysis sampling technology in freely moving rats during pr e-ischemia, incomplete ischemia and reperfusion periods induced by the occlusi on and loose of both common carotid arteries. Each dialysate sample was assayed for GABA, Tau, Glu, Cys and Arg with HPLC-electrochemical detector. Result: TGF increased the concentrations of GABA and Tau in the extracellular liquids of rat hippocampus. Compared with the model group, the concentration of Glu in the middle and large dosage groups of TGF, during the 1 20 min of ischemia, reduced by 38.64% and 31.35%, Tau increased by 13.99% a nd 12.86%, GABA advanced 25.89% and 33.99%, Cys decreased by 40.93% and 42 .08%, Arg raised to 116.95% and 108.96%, respectively. After 120 min of re perfusion, the concentration of Glu decreased by 14.55% and 11.48%, Tau incr eased by 16.13% and 14.03%, GABA increased by 24.41% and 26.22%, respectiv ely. Conclusion: TGF can increase the concentration of i nhibitory amino acids in hippocampus extracellular liquids of rats and inhibit t he excessive release of excitatory amino acids and raise the concentration of th e inhibitory amino acids and Arg during the ischemia-reperfusion periods. Ther efore, TGF can play the neuroprotective role. [

关键词(KeyWords): 缺血再灌;海马;微透析;氨基酸;神经递质
ischemia-reperfusion; hippocampus; micro dialysis; amino-acids; neurotransmitter

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金资助项目 (3 0 171185 ) ;; 北京自然科学基金资助项目 (70 12 0 3 3 )

作者(Author): 张春颖,杜贵友,王巍,叶祖光,王丹巧,孙晓芳,赵德忠
ZHANG Chun-ying1, DU Gui-you1, WANG Wei2, YE Zu-guang3 , WANG Dan-qiao2, SUN Xiao-fang2, ZHAO De-zhong2 (1. Institute of Chinese Materia Medica, China Academy of Traditio nal Chinese Medicine, Beijing 100700, China; 2. Geriatrics Institute, Xiyuan Hospital, China Academy of Traditional Chines e Medicine, Beijing 100091, China; 3. State Food and Drug Administration, Beijing 100038, China) [

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