中国中药杂志

2015, v.40(23) 4680-4685

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基于ITS及atpB-rbcL等多片段鉴定粗壮秦艽及其近缘种
DNA-based identification of Gentiana robusta and related species

熊波;赵志礼;倪梁红;嘎务;米玛;
XIONG Bo;ZHAO Zhi-li;NI Liang-hong;GAAWE Dorje;MI Ma;School of Pharmacy,Shanghai University of Traditional Chinese Medicine;Tibetan Traditional Medical College;

摘要(Abstract):

传统藏药"解吉"主要基原植物之一粗壮秦艽Gentiana robusta分布区广,且与近缘种呈同域分布现象,遗传背景复杂。多居群平行取样,直接测定该物种及其近缘物种麻花艽G.straminea、粗茎秦艽G.crassicaulis、长梗秦艽G.waltonii,外类群椭圆叶花锚Halenia elliptica的r DNA ITS区序列及叶绿体DNA的mat K,rbc L,rpo C1,trn L(UAA),psb A-trn H,atp B-rbc L,trn S(GCU)-trn G(UCC),rpl20-rps12,trn L(UAA)-trn F(GAA)序列228条;TA克隆法测定粗壮秦艽ITS区序列120条。对粗壮秦艽进行基因分型,形态学及DNA分子证据显示其可能为一杂交物种;克隆序列中寻找稳定位点,多片段组合可鉴定粗壮秦艽及其近缘种。该文对遗传背景多样的药用植物鉴定研究具有重要参考价值。
The alpine plant Gentiana robusta is an endemic species to the Sino-Himalayan subregion. Also,it is one of the original plants used as traditional Tibetan medicine Jie-Ji. We sequence the nuclear ribosomal internal transcribed spacer( ITS) regions,mat K,rbc L,rpo C1,trn L( UAA),psb A-trn H,atp B-rbc L,trn S( GCU)-trn G( UCC),rpl20-rps12,trn L( UAA)-trn F( GAA) fragments of cp DNA in both G. robusta and such relative species as G. straminea,G. crassicaulis and G. waltonii. With Halenia elliptica as the outgroup,molecular systematic analysis reveals that G. robusta is a natural hybrid. G. straminea is the mother of hybrids,but the father is not very clear. In addition,the molecular markers for distinguishing G. robusta from the parental species or closely related species are identified,respectively. Our studies may provide valuable reference for the species identifications of medicinal plants with complex genetic backgrounds.

关键词(KeyWords): 粗壮秦艽;ITS序列;自然杂交;遗传多样性;叶绿体DNA;分子鉴定
Gentiana robusta;ITS;hybrids;genetic diversity;chloroplast DNA;DNA-based identification

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基金项目(Foundation): 国家自然科学基金项目(81173654)

作者(Author): 熊波;赵志礼;倪梁红;嘎务;米玛;
XIONG Bo;ZHAO Zhi-li;NI Liang-hong;GAAWE Dorje;MI Ma;School of Pharmacy,Shanghai University of Traditional Chinese Medicine;Tibetan Traditional Medical College;

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