近红外光谱技术及其在中药道地性研究中的应用Near infrared spectroscopy (NIRS) technology and its application in geoherbs
郭兰萍;黄璐琦;Christian W Huck;
GUO Lanping1,HUANG Luqi1,CHRISTIAN W Huck2(1.Institute of Chinese Materia Medica,China Academy of Chinese Medicinal Sciences,Beijing 100700,China;2.Institue of Analytical Chemistry and Radiochemistry,University of Innsbruck,Innsbruck 6020,Austria)
摘要(Abstract):
作者在介绍了近红外光谱化学原理、光学原理、信息学原理及化学计量学分析的基础上,分析了近红外光谱分析的特点及道地性研究和道地药材鉴别的难点,进而指出近红外光谱的全息性特征,使其在道地性研究中具有极大优势。重点以道地药材的近红外鉴别和道地性研究为目标,借鉴农牧业、食品、烟酒等其他行业的研究成果,分析并阐明了近红外光谱在道地药材鉴别和评价,道地药材生境研究及评价,道地药材遗传特异性研究方面应用的现实性。并讨论了模型的可靠性、适用性和可推广性几个近红外光谱在道地药材研究应用中的关键问题,为道地性现代诠释及道地药材鉴别提出了新思路和新方法。
This review introduce of new technique and method for geoherbs analysis.After reviewing the chemical principle,optical principle and the chemometer of near infrared spectroscopy(NIRS),it analyzed the characteristic of NIRS and the difficulties of geoherbs analysis,and pointed out that NIRS had great advantage in geoherbs analysis for its full information.Referring the outcomes of NIRS in food industry,agriculture products,wines,forages,etc,it emphasized the utilization of NIRS in geoherbs,which may mainly focus on identification,and genetic characters research,as well as habitat evaluation of geoherbs.At finally,it discussed some key points which may determine NIRS utilization in geoherbs,such as reliability,applicability,and popularity.
关键词(KeyWords):
近红外光谱;道地药材;鉴别;道地性
near infrared spectroscopy(NIRS);geoherbs;identification;geoherbism
基金项目(Foundation): 国家自然科学基金项目(30672616);; 国家重点基础研究发展计划(973)项目(2006CB504700);; 国家中医药管理局行业专项项目(200707014);; 国家“十一五”科技支撑计划项目(2006BAI09B03);; 国家中医药管理局中医药标准化项目(ZYYS-2008)
作者(Author):
郭兰萍;黄璐琦;Christian W Huck;
GUO Lanping1,HUANG Luqi1,CHRISTIAN W Huck2(1.Institute of Chinese Materia Medica,China Academy of Chinese Medicinal Sciences,Beijing 100700,China;2.Institue of Analytical Chemistry and Radiochemistry,University of Innsbruck,Innsbruck 6020,Austria)
Email:
DOI:
参考文献(References):
- [1]黄璐琦,郭兰萍,胡娟,等.道地药材的分子机制及其遗传基础[J].中国中药杂志,2008,33(20):2303.
- [2]黄璐琦.分子生药学[M].2版.北京:北京大学医学出版社,2006:9.
- [3]Siesler HW,Ozaki Y,Kawata S,et al.Near infrared spectros-copy:Principle instruments and application[M].Weinheim:Wiley-VCH,2002.
- [4]Huck C W.Advances of near-infrared spectroscopy in phyto-chemistry[J].Near Infrared Spectrosc,2002,10:491.
- [5]周帼雄.美、英、欧三部药典近红外光谱分析方法概述[J].中国药品标准,2003,4(2):6.
- [6]王宁,武卫红.近红外光谱技术在中药分析领域中的应用[J].山东中医药大学学报,2007,31(4):350.
- [7]邢志娜,张达磊.近红外光谱分析技术及其在中药领域的应用进展[J].时珍国医国药,2005,16(5):416.
- [8]Huck C W,Maurer R,Bonn G K,et al.Quality control of liquidplant extracts in the phytopharmaceutical industry[J].Near In-frared Spectrosc,1999,9:487.
- [9]Ciurczak E W,Drennen J K.Pharmaceutical and medical appli-cations of near-infrared spectroscopy[M].New York:MarcelDekker Inc,2002.
- [10]王平,谢洪平,陈泽琴,等.中药材人参的道地性差异的近红外光谱研究[J].苏州大学学报:医学版,2004,24(5):648.
- [11]王钢力,石岩,魏玉海,等.近红外光谱鉴别冬虫夏草道地药材[J].中草药,2006,37(10):1569.
- [12]Esbensen Klm H.Multivariate data analysis in practice[M].5thedition.Norway:CAMO Processs As,2005.
- [13]黄璐琦,郭兰萍,华国栋.道地药材属性及研究对策[J].中国中医药信息杂志,2007,14(2):44.
- [14]Slaton M R,Hunt E R,Smith W K.Estimating near-infraredleaf reflectance fromleaf structural characteristics[J].AmJ Bot,2001,88:278.
- [15]范积平,张贞良,张柳瑛,等.近红外光谱法测定药用大黄中4种蒽醌类成分[J].第二军医大学学报,2005,26(10):1194.
- [16]史春香,杨悦武,郭治昕,等.近红外技术定量分析丹参药材[J].中药材,2006,29(9):897.
- [17]刘浩,黄艳萍,相秉仁,等.黄花蒿中秦好苏的近红外光谱测定[J].中国医药工业杂志,2007,38(8):584.
- [18]臧鹏,陈斌,严衍禄.短波近红外光谱法测定葛根中总异黄酮含量[J].时珍国医国药,2007,18(1):2614.
- [19]王东,王玲.近红外光谱法测定黄芩药材中黄芩苷的含量[J].中医研究,2007,20(2):20.
- [20]Shao YN,He Y,G幃mez AH,et al.Visible/near infrared spec-trometric technique for nondestructive assessment of tomato‘Heatwave’(Lycopersicum esculentum)quality characteristics[J].J Food Eng,2007,81(4):672.
- [21]G幃mez A H,He Y,Pereira A G.Non-destructive measurementof acidity,soluble solids and firmness ofSatsuma mandarinusingVis/NIR-spectroscopy techniques[J].J Food Eng,2006,77(2):313.
- [22]McGlone V A,Kawano S.Firmness,dry-matter and soluble-sol-ids assessment of postharvest kiwifruit by NIR spectroscopy[J].Postharvest Bio Tech,1998,13(2):131.
- [23]Nicolai B M,Verlinden B E,Desmet M,et al.Time-resolvedand continuous wave NIR reflectance spectroscopy to predict solu-ble solids content and firmness of pear[J].Postharvest BioTech,2008,47(1):68.
- [24]Cayuela J A.Vis/NIR soluble solids prediction in intact oranges(Citrus sinensisL.)cv valencia late by reflectance[J].Posthar-vest Bio Tech,2008,47(1):75.
- [25]Bureau S,Ruiz D,Reich M,et al.Rapid and non-destructiveanalysis of apricot fruit quality using FT-Near-Infrared spectrosco-py[J].Food Chemistry,2009,115(3):1133.
- [26]Heigl N,Huck C W,Rainer M,et al.Near infrared spectrosco-py,cluster and multivariate analysis hyphenated to thin layerchromatography for the analysis of amino acids[J].AminoAcids,2006,31:45.
- [27]Huck C W,Maurer R,Popp M,et al.Quantitative fourier trans-form near infrared reflectance spectroscopy(NIRS)compared tohigh performance liquid chromatography(HPLC)of a flavone inflosPrimulae verisextractives[J].Pharm Pharmacol Lett,1999,9(1):26.
- [28]Guggenbichler W,Huck C W,Kobler A,et al.Near infrared re-flectance spectroscopy as a tool for quality control in wine produc-tion[J].J Food Agric Environm,2006,4(2):98.
- [29]Norris HK,Hart J R.Direct spectrophotometric determination ofmoisture content of grain and seeds[J].J Near Infrared Spec-trosc,1996,4:23.
- [30]Tsenkova R,Atanassova S,Toyoda K.Near-infrared spectrosco-py for dairy management:measurementof unhomogenized milkcomposition[J].J Dairy Sci,1999,82:2344.
- [31]Kays S E,Shimizu N,Barton F E,et al.Near-infrared transmis-sion and reflectance spectroscopy for the determination of dietaryfiber in barley cultivars[J].Crop Sci,2005,45:2307.
- [32]Smith TN,Pesti G M,Bakalli R I,et al.The use of near-infra-red reflectance spectroscopy to predict the moisture,nitrogen,calcium,total phosphorus,gross energy,and phytate phosphoruscontents of broiler excreta[J].Poultry Sci,2001,80(3):314.
- [33]Welle R,Greten W,Rietmann B,et al.Near-infrared spectros-copy on chopper to measure maize forage quality parameters on-line[J].Crop Sci,2003,43:1407.
- [34]Berzaghi P,Zotte AD,Jansson LM,et al.Near-infrared reflec-tance spectroscopy as a method to predict chemical composition ofbreast meat and discriminate between different n-3 feedingsources[J]Poultry Sci,2005,84(1):128.
- [35]白雁,贾永,王东,等.应用近红外漫反射光谱技术测定酒炖熟地黄中的还原糖含量[J].中药材,2006,29(10):1035.
- [36]宋丽丽,范丙义,徐晓杰,等.近红外光谱法用于六味地黄丸模拟样品中熊果酸的含量测定[J].中国中药杂志,2006,31(10):1590.
- [37]Chang C W,Laird D A,Mausbach MJ,et al.Near-infrared re-flectance spectroscopy-principal components regression analyses ofsoil properties[J].Soil Sci Soc Am J,2001,65:480.
- [38]Cozzolino D,Moron A.The potential of near-infrared reflectancespectroscopy to analyse soil chemical and physical characteristics[J].J Agric Sci,2003,140:65.
- [39]Ben-Dor E,Banin A.Near infrared analysis(NIRA)as a rapidmethod to simultaneously evaluate several soil properties[J].SoilSci Soc Am J,1995,59:364.
- [40]Siebielec G,McCarty G W,Stuczynski TI,et al.Near and mid-infrared diffuse reflectance spectroscopy for measuring soil metalcontent[J].J Environ Qual,2004,33:2056.
- [41]Mouazen A M,Karoui R,Baerdemaeker J D,et al.Character-ization of soil water content using measured visible and near infra-red spectra[J].Soil Sci Soc Am J,2006,70:1295.
- [42]Couillard A,Turgeon AJ,Shenk J S,et al.Near infrared reflec-tance spectroscopy for analysis of turf soil profiles[J].Crop Sci,1997,37:1554.
- [43]Waiser TH,Morgan C L S,Brown D J,et al.In situcharacter-ization of soil clay content with visible near-infrared diffuse reflec-tance spectroscopy[J].Sci Soc Am J,2007,71:389.
- [44]Bacchus S T,Archibald D D,Brook G A,et al.Near-infraredspectroscopy of a hydroecological indicator:Newtool for determi-ning sustainable yield for Floridan Aquifer system[J].HydrolProcess,2003,17(9):1785.
- [45]Cohen MJ,Prenger J P,DeBusk W F.Visible-near infrared re-flectance spectroscopy for rapid,nondestructive assessment of wet-land soil quality[J].J Environ Qual,2005,34:1422.
- [46]Vaidyanathan S,Harvey LM,Brian M.Deconvolution of near-in-frared spectral information for monitoring mycelial biomass andother key analytes in a submerged fungal bioprocess[J].AnalChim Acta,2001,428(1):41.
- [47]Ros啨n P.Near-infrared spectrometry(NIRS):Anewtool for in-ferring past climatic changes from lake sediments[J].Holocene,2000,10(2):161.
- [48]Vetter S,Franz C,Giasl S,et al.Inheritance of sesquiterpenelactone types within theAchillea millefoliumcomplex(Composi-tae)[J].Plant Breeding,1996,116(1):79.
- [49]Fischer U,Vetter S,Novak J,et al.Inheritance of the sesquiter-penes longipinenone and hydroxy-longipinenone within theAchill-ea millefoliumcomplex(Compositae)[J].Plant Breed,2001,120:251.
- [50]Fonseca C E L,Hansen J L,Thomas E M,et al.Near infraredreflectance spectroscopy prediction and heritability of neutral de-tergent-soluble fiber in alfalfa[J].Crop Sci,1999,39(5):1265.
- [51]Velasco L,P啨rez-Vich B,Fern偄ndez-Mart姫nez J M.Use of near-infrared reflectance spectroscopy for selecting for high stearic acidconcentration in single husked achenes of sunflower[J].CropSci,2004,44:93.
- [52]Tillmana B L,Gorbeta D W,Personb G.Predicting oleic andlinoleic acid content of single peanut seeds using near-infrared re-flectance spectroscopy[J].Crop Sci,2006,46:2121.
- [53]Blosser TH,Reeves J B,Bond III J.Factors affecting analysis ofthe chemical composition of tall fescue with near infrared reflec-tance spectroscopy[J].J Dairy Sci,1988,71(2):398.
- [54]Reeves J B.Near Infrared reflectance spectroscopic analysis ofsodium chlorite-treated forages and other plant materials[J].JDairy Sci,1988,71(2):14.
- [55]Marten G C,Brink G E,Buxton D R,et al.Near infrared re-flectance spectroscopy analysis of forage quality in fourLegumespecies[J].Crop Sci,1984,24:1179.
- [56]P ADuncan,D C Clanton,D HClark.Near infrared reflectancespectroscopy for quality analysis of sandhills meadow forage[J].J Anim Sci,1987,64:1743.
- [57]Welle R,Greten W,Rietmann B,et al.Near-infrared spectros-copy on chopper to measure maize forage quality parameters on-line[J].Crop Sci,2003,43:1407.
- 郭兰萍
- 黄璐琦
- Christian W Huck
GUO Lanping1- HUANG Luqi1
- CHRISTIAN W Huck2(1.Institute of Chinese Materia Medica
- China Academy of Chinese Medicinal Sciences
- Beijing 100700
- China
- 2.Institue of Analytical Chemistry and Radiochemistry
- University of Innsbruck
- Innsbruck 6020
- Austria)
- 郭兰萍
- 黄璐琦
- Christian W Huck
GUO Lanping1- HUANG Luqi1
- CHRISTIAN W Huck2(1.Institute of Chinese Materia Medica
- China Academy of Chinese Medicinal Sciences
- Beijing 100700
- China
- 2.Institue of Analytical Chemistry and Radiochemistry
- University of Innsbruck
- Innsbruck 6020
- Austria)