中国中药杂志

2004, (07)

[打印本页] [关闭]
本期目录(Current Issue) | 过刊浏览(Past Issue) | 高级检索(Advanced Search)

云母对大鼠慢性萎缩性胃炎干预及逆转治疗的机理探讨
Mechanism of isinglass in prevention and treatment of chronic atrophic gastritis in rats

姒健敏,徐妙,王良静,王海云,吴加国,曹倩
SI Jian-min, XU Miao, WANG Liang-jing, WANG Hai-yun, WU Jia-guo, CAO Qian (Gastroenterologic Laboratory of Clinical Institute, Sir Run Run Shaw Hospital Affiliated to Medical School of Zhejiang University, Hangzhou 310016, China)

摘要(Abstract):

目的 :研究云母对大鼠慢性萎缩性胃炎的干预及逆转治疗的机理。方法 :以 6 0 %的乙醇 ,2 0mmol·L-1的去氧胆酸钠和 0 .1%的氨水在 2 4周建立SD大鼠慢性萎缩性胃炎模型。应用硫糖铝和 2种剂量的云母进行干预及逆转治疗后 ,测定鼠胃黏膜细胞增殖带、血清表皮生长因子及生长激素水平。结果 :高、低云母干预组及高云母逆转组胃黏膜细胞增殖带与正常组接近 (P >0 .0 5 ) ,而明显高于模型组 (P <0 .0 1) ;低云母逆转组胃黏膜细胞增殖带低于正常组 (P <0 .0 1) ,但高于模型组 (P <0 .0 1)。高、低云母干预及逆转组血清表皮生长因子明显高于正常组 (P <0 .0 1)及模型组 (P <0 .0 5 ) ;而血清生长激素各组间无明显差别 (P >0 .0 5 )。结论 :云母干预及逆转治疗大鼠慢性萎缩性胃炎的作用与刺激内源性的表皮生长因子分泌、促进上皮增殖修复有关。
Objective: To investigate the mechanism of isinglass in the prevention and treatment of chronic atrophic gastritis (CAG) in rats. Method: Animal models of SD rats with CAG were made in accordance with the previous experience of combined administration of 60% ethanol, 20 mmol·L -1 sodium deoxycholate and 0.1% ammonia water. In prevention groups, sucralfate and isinglass were used as preventive therapy while CAG rat model was being made. In the reverse groups, sucralfate and isinglass were used to treat rats after establishment of CAG rat model. Finally all the rats were executed . Then the length of the proliferation zone of the gastric mucosa and serum epidermal growth factors(EGF) and growth hormones (GH)level were studied. Result: In isinglass prevention groups and high dose isinglass reverse group, the length of the proliferation zone of the gastric mucosa was very close to that in normal control group (P>0.05), much better than model control group (P<0.01). In low dose isinglass reverse group, it was lower than that in normal control group (P<0.01), but much better than model control group (P<0.01). In both prevention and reverse groups, serum EGF level was higher than that in normal (P<0.01)and model control group(P<0.05). Serum GH level was the same in every group (P>0.05). Conclusion: The mechanism of isinglass in the prevention and treatment of CAG rats lies in revitalizing and proliferating gastric mucosal cells by stimulating endogenous EGF secretion.

关键词(KeyWords): 云母;慢性萎缩性胃炎;SD大鼠;增殖细胞核抗原;表皮生长因子;生长激素
isinglass; CAG; SD rat; proliferating cell nuclear antigen; EGF; GH

Abstract:

Keywords:

基金项目(Foundation): 浙江省科技厅自然科学基金项目 ( 0 1110 3 0 18) ;; 杭州市科技发展计划项目 ( 2 0 0 2 12 1A15 )

作者(Author): 姒健敏,徐妙,王良静,王海云,吴加国,曹倩
SI Jian-min, XU Miao, WANG Liang-jing, WANG Hai-yun, WU Jia-guo, CAO Qian (Gastroenterologic Laboratory of Clinical Institute, Sir Run Run Shaw Hospital Affiliated to Medical School of Zhejiang University, Hangzhou 310016, China)

Email:

DOI:

扩展功能
本文信息
服务与反馈
本文关键词相关文章
本文作者相关文章
中国知网
分享